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Plasmid#193577PurposeExpresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a flexible linker of 6 amino acids in mammalian cells.DepositorInsertClathrin light chain B (CLTB Human)
UseTagsmEGFPExpressionMammalianMutationDeleted amino acids 207-211PromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
CLC-EGFP206(flex7)
Plasmid#193578PurposeExpresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a flexible linker of 7 amino acids in mammalian cells.DepositorInsertClathrin light chain B (CLTB Human)
UseTagsmEGFPExpressionMammalianMutationDeleted amino acids 207-211PromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
CLC-pmCherryC1
Plasmid#27680DepositorInsertClathrin Light Chain (Clta Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
GST-auxilin(547-910)
Plasmid#62572PurposeCodes for a GST-auxilin fusion protein that is functional as a co-factor in hsc70-dependent clathrin coat disruptionDepositorInsertAuxilin
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST44-AP-1 core
Plasmid#184809PurposeBacterial expression of clathrin adaptor AP-1 core complexDepositorInsertHs AP1S3; Hs AP1B1 (1-584); Mm AP1G1 (1-595); Mm AP1M1
UseTagsGST tag and His6ExpressionBacterialMutationAP1S3: S148C; AP1B1: K359R, E476KPromoterT7Available sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
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μ2-HA-D176A
Plasmid#32754DepositorInsertμ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
UseTagsHAExpressionMammalianMutationD176APromoterCMVAvailable sinceNov. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
CRY2olig-mCh-CLC
Plasmid#60035PurposeExpresses a fusion of CRY2olig (PHR, E490G) with mCherry and mouse clathrin light chainDepositorInsertCRY2-mCh-Clathrin Light Chain (CRY2 Mouse, Mustard Weed)
UseTagsExpressionMammalianMutationM354I, E490G CRY2, A9D*PromoterCMVAvailable sinceOct. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
FKBP-alpha(740-977)-GFP
Plasmid#100731PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosis (negative control)DepositorInsertAP2A1 (Ap2a1 Mouse)
UseTagsFKBP and GFPExpressionMammalianMutationLong isoformPromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP-GTSE1(638-720)-GFP
Plasmid#100735PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertGTSE1 (GTSE1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationC-terminus onlyPromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta2(616-951)-GFP
Plasmid#100726PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationLong isoformPromoterCMVAvailable sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta1(617-949)-GFP
Plasmid#100732PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP1B1 (AP1B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta3(702-1094)-GFP
Plasmid#100734PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP3B1 (AP3B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationappendage and ear onlyPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta2(616-951)-mRuby2
Plasmid#100730PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsFKBP and mRuby2ExpressionMammalianMutationLong isoformPromoterCMVAvailable sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
PDZb-beta2(616-951)-mCherry
Plasmid#100736PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsPDZb and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
Syndapin2-pmCherryC1
Plasmid#27681DepositorInsertSyndapin 2
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-FCHO1
Plasmid#196910Purposevisualization of clathrin-coaed vesicle formation in living mammalian cellsDepositorInsertFCHO1 (FCHO1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-CLCb (EED/QQN)
Plasmid#47422DepositorInsertCLCb EED/QQN (CLTB Human)
UseTagsEGFPExpressionMammalianMutationEED changed to QQN, in aa20-41 regionPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta2(616-951)-GFP Y215A
Plasmid#100727PurposeTo express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationLong isoform Y215A mutation (Y815A in full length)PromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-beta2(616-951)Double
Plasmid#100746PurposeBacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutantDepositorInsertAP2B1 (AP2B1 Human)
UseTagsGSTExpressionBacterialMutationLong isoform with deletion of clathrin binding mo…PromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only