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LentiCRISPR-sgC17orf89-2

Plasmid

#86135

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting C17orf89

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA targeting C17orf89 (NDUFAF8 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pGEMHE-TNKS-2

Plasmid

#154149

Purpose

For making TNKS-2 RNA

Depositor

Carmen Williams

Article

Gambini et al Dev Cell. 2020 Jun 8;53(5):545-560.e7. d

Insert

Tankyrase 2 (TNKS2 Human)

Use

In vitro transcription

Tags

FLAG

Expression

Mutation

Promoter

T7

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgSHOC2-2

Plasmid

#86129

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting SHOC2

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA 2 targeting SHOC2 (SHOC2 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2017

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgC1orf27-2

Plasmid

#86131

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting C1orf27

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA 2 targeting C1orf27 (C1orf27 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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PXPR007 sgCIC-2

Plasmid

#74959

Purpose

Cas9 + sgCIC-2 with blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

CIC (CIC Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-sgCD71-2

Plasmid

#46918

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CD71 gene

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

sgCD71 -2 (TFRC Human)
Puromycin resistance and mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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PXPR007 sgATXN1L-2

Plasmid

#74963

Purpose

Cas9 + sgATXN1L-2 with blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ATXN1L (ATXN1L Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pGS-CD64-2

Plasmid

#109191

Purpose

Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

Full-length engineered CD64, derived from human CD64

Use

Tags

Twin-Strep

Expression

Insect

Mutation

engineered for efficient expression, see publicat…

Promoter

Polyhedrin

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgCIC-2

Plasmid

#74967

Purpose

sgCIC-2, puromycin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

CIC (CIC Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgATXN1L-2

Plasmid

#74971

Purpose

sgATXN1L-2, puromycin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ATXN1L (ATXN1L Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-sgCXCR4-2

Plasmid

#46917

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CXCR4 gene

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

sgCXCR4 -2 (CXCR4 Human)
Puromycin resistance and mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pBS-elav-2

Plasmid

#26131

Depositor

Phillip Newmark

Article

Wang et al Genes Dev. 2010 Sep 15. 24(18):2081-92.

Insert

Smed-elav2

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pPR244-elav-2

Plasmid

#26144

Depositor

Phillip Newmark

Article

Wang et al Genes Dev. 2010 Sep 15. 24(18):2081-92.

Insert

Smed-elav2

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP×2-N1

Plasmid

#86775

Purpose

A mammalian expression vector with double EGFP at C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pPtet-2-VP16

Plasmid

#213842

Purpose

Used for expressing VP16 fused to ZF-2.

Depositor

James Collins

Article

Alcantar et al Molecular Cell, June 2024

Insert

VP16

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMOR22-2-ISH

Plasmid

#15839

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

MOR22-2 (Olfr69 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pMOR31-2-ISH

Plasmid

#15844

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

MOR31-2 (Olfr690 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pBS Coupler 2

Plasmid

#185825

Purpose

Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].

Depositor

Atsushi Miyawaki

Article

Shimozono et al Methods Cell Biol. 2008;85:381-93. doi:

Insert

(GlyGlyGlyGlySer)x3 linker

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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Human ICAM-2

Plasmid

#8634

Depositor

Timothy Springer

Article

Staunton et al Nature 1989 May 4;339(6219):61-4.

Insert

ICAM-2 (ICAM2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-2

Plasmid

#17992

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-2 (Wnt2 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only