-
Plasmid#159942Purposeoptical regulation of nuclear exportDepositorInsertNLS-YPET-optoNES
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-mScarlet-optoNES-WPRE
Plasmid#159943Purposeoptical regulation of nuclear exportDepositorInsertNLS-mScarlet-optoNES
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-mScarlet-C1-WPRE
Plasmid#159955Purposeuntargeted markerDepositorInsertmScarlet-C1
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-Dronpa-optoNES-WPRE
Plasmid#159944Purposeoptical regulation of nuclear exportDepositorInsertNLS-Dronpa-optoNES
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-mTurquoise2-3XNLS-optoNES-WPRE
Plasmid#159946Purposeoptical regulation of nuclear exportDepositorInsertmTurquoise2-3XNLS-optoNES
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-NLS-mTurquoise2-optoNES-WPRE
Plasmid#159962Purposeoptical regulation of nuclear exportDepositorInsertNLS-mTurquoise2-optoNES
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterCMV-IEAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-mTurquoise2-optoJNKi-V416I-WPRE
Plasmid#159970Purposeoptogenetic JNK inhibitor with extended relaxation timeDepositorInsertmTurquoise2-optoJNKi-V416I
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-mTurquoise2-optoJNKi-C450A-WPRE
Plasmid#159969Purposenegative control (non-activatable) optogenetic JNK inhibitorDepositorInsertmTurquoise2-optoJNKi-C450A
UseAdenoviralTagsExpressionMammalianMutationC450APromoterhSyn1Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES.F434L-WPRE
Plasmid#159975Purposeoptical regulation of nuclear export with shorter relaxation timeDepositorInsertNLS-YPET-optoNES.F434L
UseAdenoviralTagsExpressionMammalianMutationF434LPromoterhSyn1Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES.V416I-WPRE
Plasmid#159973Purposeoptical regulation of nuclear export with extended relaxation timeDepositorInsertNLS-YPET-optoNES.V416I
UseAdenoviralTagsExpressionMammalianMutationV416IPromoterhSyn1Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES.I427V-WPRE
Plasmid#159974Purposeoptical regulation of nuclear export with shorter relaxation timeDepositorInsertNLS-YPET-optoNES.I427V
UseAdenoviralTagsExpressionMammalianMutationI427VPromoterhSyn1Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-OptoPB
Plasmid#113935PurposeExpress a LOV2 fusion protein to enable light-dependent cytosol-to-PM translocation and dissect protein-phosphoinositide interactionsDepositorInsertOptoPB
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RBP-GFP(S45C)-ZdK1
Plasmid#185791PurposeEncodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linkerDepositorInsertRBP-sfGFP(S45C)-ZdK1
UseTags10xHis, TEV protease cleavage site after His TagExpressionBacterialMutationSer to Cys mutation at start of GFP, residue 45 i…PromoterT7Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Opto-CRAC version 1
Plasmid#101245PurposeGenetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signalingDepositorInsertLOV2 and STIM1 cytosolic fragment
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-PA-Rac1-C450A
Plasmid#22028PurposeExpression of Rac1 fused with light-insensitive LOV2 (C450A mutation)DepositorInsertPA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
UseTags6xHis and mCherryExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
CMV-CASANOVA
Plasmid#113035PurposeCASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9DepositorInsertCASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-PA-Rac1-C450A
Plasmid#22021PurposeExpression of Rac1 fused with light-insensitive LOV2 (C450A mutation)DepositorInsertPA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
UseTags6X His and mVenusExpressionBacterial, Insect, and Mamm…MutationLOV domain contains the light-insensitive C450A m…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-CASANOVA
Plasmid#113036PurposeAAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9DepositorInsertCASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOVSD
Plasmid#81033PurposeMammalian expression of the super-dark state of LOV2DepositorInsertLOVSD
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationC450A, L514K, G528A, L531E, N538EPromoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PA(WT)-Vav2
Plasmid#86977PurposePI(DM): Photo-activatable/light sensitiveDepositorInsertsUseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only