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Plasmid#191321PurposeExpresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV lightDepositorInsertTUBB2a-Dronpa (Tubb2a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDronpa-N1-Tau-μDpTT
Plasmid#182323PurposeMicrotubule-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertTau-μDronpa-TagRFP-T
UseTagsMAP tauExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lifeact-Dronpa
Plasmid#182330PurposeActin-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.DepositorInsertLifeact-Dronpa
UseTagsN-terminal 17 amino acids from budding yeast Abp1…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CBX1mut-Dronpa
Plasmid#138250PurposeTriple-mutant CBX1 chromodomain (residues 20-73) tagged with reversibly switchable FP Dronpa. Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.DepositorInsertCBX1mut-Dronpa
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Alpha-0 Dronpa
Plasmid#44193DepositorInsertG protein alpha 0 (Gnao1 Rat)
UseTagsDronpaExpressionMammalianMutationIntroduced KpnI and SpeI sites between 92nd and 9…PromoterCMVAvailable sinceApril 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-Dronpa-optoNES-WPRE
Plasmid#159944Purposeoptical regulation of nuclear exportDepositorInsertNLS-Dronpa-optoNES
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-rsGreenF-E-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-Skylan62A-2A-YFP-SLBP
Plasmid#205762PurposeSingle-color FUCCI4 senor for transitions of all four cell cylce phasesDepositorInsertrsGFE-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-SK62A-2A-YFP-SLBP
UseLentiviralTagsExpressionMammalianMutationwtPromoterAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIA027
Plasmid#200418PurposeamyE::(dronPA cm), for dronPA fusions. PALM-compatible integration vector for Bacillus subtilis. Includes dronPA photoswitchable FP with multi cloning sites (MCS) for fast and interchangeable cloning.DepositorArticleTypeEmpty backboneUseTagsdronPAExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIA035
Plasmid#200426PurposeamyE::(Ppen-lacIΔ11-dronPA cm). PALM integration vector includes dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.DepositorArticleInsertLacI
UseTagsdronPAExpressionBacterialMutationPromoterPpenAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DAKAP-μDpTT
Plasmid#182324PurposeMitochondria-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertDAKAP-μDronpa-TagRFP-T
UseTagsN-terminal 30 amino acids from DAKAP1ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-μDpTT
Plasmid#182321PurposePlasma membrane-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertLyn-μDronpa-TagRFP-T
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CYP450-μDpTT
Plasmid#182325PurposeEndoplasmic reticulum-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertDAKAP-μDronpa-TagRFP-T
UseTagsN-terminal 27 amino acids from CYP450ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lifeact-μDpTT
Plasmid#182322PurposeActin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertLifeact-μDronpa-TagRFP-T
UseTagsN-terminal 17 amino acids from budding yeast Abp1…ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DrFLINC-AKAR-Kras
Plasmid#182334PurposeGenetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.DepositorInsertDrFLINC-AKAR
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIIα-μDpTT
Plasmid#182327PurposePKA RIIα subunit-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertRIIalpha-μDronpa-TagRFP-T (PRKAR2A Human)
UseTagsFull-length human PKA type 2alpha regulatory subu…ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-H2B-μDpTT
Plasmid#182328PurposeHistone H2B-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertH2B-μDronpa-TagRFP-T (H2BC3 Human)
UseTagsFull-length human histone H2BExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-β2AR-μDpTT
Plasmid#182326PurposeBeta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertbeta2AR-μDronpa-TagRFP-T (ADRB2 Human)
UseTagsFull-length human beta2 adrenergic receptorExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJB089
Plasmid#98914PurposeExpress Dronpa tagged ZapA and pAmCherry1 tagged FtsZ in E coliDepositorInsertsFtsZ
ZapA
UseTagsDronpa and pAmCherry1ExpressionBacterialMutationPromoterT5-lacAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJB057
Plasmid#98911PurposeExpress Dronpa tagged ZapA in E coliDepositorInsertZapA
UseTagsDronpaExpressionBacterialMutationPromoterT5-lacAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJB073
Plasmid#98912PurposeExpress Dronpa tagged ZapB in E coliDepositorInsertZapB
UseTagsDronpaExpressionBacterialMutationPromoterT5-lacAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only