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Showing: 81 - 100 of 113381 results

  1. pKM197

    Plasmid
    #132665
    Purpose
    pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.
    Depositor
    Joseph Sorg
    Article
    Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:
    Insert
    Upstream & Downstream pyrE deletion region
    Use
    E. coli - c. difficile shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132665-plasmid-map-sequence-id-259679

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  5. pJC.15A.sgRNA.TA

    Plasmid
    #232483
    Purpose
    sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.
    Depositor
    James Collins
    Article
    Collins et al Microbiology Resource Announcements
    Inserts
    Toxin-antitoxin System from CD630
    medium-copy-number p15A origin of replication
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P3 constitutive promoter and native promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    232483-plasmid-map-sequence-id-466415

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  6. pJK040 (pET-PurQ-A128T)

    Plasmid
    #73840
    Purpose
    Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)
    Depositor
    JoAnne Stubbe, T. J. Kappock
    Article
    Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2
    Insert
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A128T mutant
    Promoter
    T7
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    73840-plasmid-map-sequence-id-136936

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  10. pRPF215

    Plasmid
    #106377
    Purpose
    C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon
    Depositor
    Robert Fagan, Neil Fairweather
    Article
    Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1
    Insert
    Ptet-himar1-Ter(slpA)-transposon
    Use
    E. coli - c. difficile shuttle vector
    Tags
    Expression
    Mutation
    Codon optimised for C. difficile
    Promoter
    Ptet
    Available since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    106377-plasmid-map-sequence-id-205135

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Showing: 81 - 100 of 113381 results