Skip to main content
Addgene
Showing: 201 - 250 of 358 results

  7. pcDNA3-sACE2-T92Q-sfGFP

    Plasmid
    #145178
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP glycosylation mutant T92Q
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145178-plasmid-map-sequence-id-292625

    Enlarge

  11. pEF-GalNAcT2-mGold

    Plasmid
    #218964
    Purpose
    Expresses full length GalNAcT2-mGold in mammalian cells
    Depositor
    Benjamin Glick
    Insert
    GalNAcT2-mGold (GALNT2 Human)
    Use
    Tags
    mGold
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218964-plasmid-map-sequence-id-445690

    Enlarge

  12. pEF-GalNAcT2-msYFP2

    Plasmid
    #218963
    Purpose
    Expresses full length GalNAcT2-msYFP2 in mammalian cells
    Depositor
    Benjamin Glick
    Insert
    GalNAcT2-msYFP2 (GALNT2 Human)
    Use
    Tags
    msYFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218963-plasmid-map-sequence-id-446179

    Enlarge

  22. pTXB1-FIS

    Plasmid
    #51564
    Purpose
    Expression of E. coli FIS for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    FIS
    Use
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51564-plasmid-map-sequence-id-78815

    Enlarge

  23. pTXB1-CII

    Plasmid
    #51566
    Purpose
    Expression of bacteriophage lambda CII for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    cII
    Use
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51566-plasmid-map-sequence-id-78825

    Enlarge

  25. pTXB1-CI

    Plasmid
    #51565
    Purpose
    Expression of bacteriophage lambda CI for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    cI
    Use
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51565-plasmid-map-sequence-id-78820

    Enlarge

  28. pCMVtrunc LifeactΔC4-msfGFPΔN7

    Plasmid
    #231555
    Purpose
    Mammalian expression of C-terminally truncated Lifeact fused to N-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy
    Depositor
    Manos Mavrakis
    Article
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    Lifeact (ABP140 Budding Yeast)
    Use
    Tags
    msfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMVtrunc
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231555-plasmid-map-sequence-id-459696

    Enlarge

  29. pCMVtrunc msfGFPΔC9-LifeactΔN2

    Plasmid
    #231556
    Purpose
    Mammalian expression of N-terminally truncated Lifeact fused to C-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy
    Depositor
    Manos Mavrakis
    Article
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    Lifeact (ABP140 Budding Yeast)
    Use
    Tags
    msfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMVtrunc
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231556-plasmid-map-sequence-id-459689

    Enlarge

  33. pTXB1-CRP/CAP

    Plasmid
    #51563
    Purpose
    Expression of E. coli CRP/CAP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    CRP/CAP
    Use
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51563-plasmid-map-sequence-id-78803

    Enlarge

  37. GTT-g

    Plasmid
    #81109
    Purpose
    Gene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    sfGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81109-plasmid-map-sequence-id-154333

    Enlarge

  38. GTU-g

    Plasmid
    #81094
    Purpose
    Gene Tagging vector URA/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    sfGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81094-plasmid-map-sequence-id-154249

    Enlarge

  39. GTH-g

    Plasmid
    #81104
    Purpose
    Gene Tagging vector HIS/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    sfGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81104-plasmid-map-sequence-id-154299

    Enlarge

  40. GTL-g

    Plasmid
    #81099
    Purpose
    Gene Tagging vector LEU/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    sfGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81099-plasmid-map-sequence-id-154274

    Enlarge

  42. pCMVtrunc msfGFPΔC10-beta-actin

    Plasmid
    #231548
    Purpose
    Mammalian expression of human beta actin fused to C-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy
    Depositor
    Manos Mavrakis
    Article
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    ACTB (ACTB Human)
    Use
    Tags
    msfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMVtrunc
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231548-plasmid-map-sequence-id-459700

    Enlarge

  44. pCT5-bac1.8

    Plasmid
    #119871
    Purpose
    Constitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli
    Depositor
    Claudia Schmidt-dannert
    Article
    Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p
    Inserts
    CymR'
    sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    SNP in 592 bp
    Promoter
    PserA promoter and Pveg promoter with CuO sequence
    Available since
    Jan. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119871-plasmid-map-sequence-id-232788

    Enlarge

  48. pCS2+MCS-P2A-sfGFP

    Plasmid
    #74668
    Purpose
    Plasmid for expression of wild-type or mutant cDNA fragments (mutation reporter) or complete cDNAs in frame with superfolderGFP.
    Depositor
    Jason Berman
    Insert
    sfGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74668-plasmid-map-sequence-id-146800

    Enlarge

  49. pKD072

    Plasmid
    #136472
    Purpose
    SSB-C-term-GFP fusion expression plasmid
    Depositor
    James Keck
    Article
    Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60
    Insert
    SSB-C-term-GFP (ssb E. coli)
    Use
    Tags
    superfolder GFP
    Expression
    Bacterial
    Mutation
    superfolder GFP attached to C-terminus at Phe 178
    Promoter
    T7 promoter
    Available since
    April 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    136472-plasmid-map-sequence-id-267962

    Enlarge

Showing: 201 - 250 of 358 results