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Plasmid#163752PurposegRNA expression vector to target the SOX2 stop codon for HDR gene tagging in human cells.DepositorInsertgRNA_SOX2 (SOX2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-mRuby3
Plasmid#166136PurposeGateway entry vector for mRuby3DepositorArticleInsertmRuby3
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C1 SADBs shRNA Resistant
Plasmid#67155PurposepEGFP containing N-terminally EGFP Tagged mouse SADBs resistant to an shRNADepositorInsertBrsk1 (Brsk1 Mouse)
UseTagsEGFPExpressionMammalianMutationnucleotide 294 C-T, nucleotide 297 C-GPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTER SADB shRNA Human
Plasmid#67151PurposeDoxycycline-regulated mammalian expression vector for expressing shRNA against human SADBDepositorInsertBRSK1 (BRSK1 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1 TetAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-ccdB
Plasmid#166145PurposeGateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.DepositorArticleTypeEmpty backboneUseTagsHis10ExpressionBacterialMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_ACh3.0
Plasmid#121922Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0DepositorHas ServiceAAV9InsertGPCR activation based ACh sensor GRAB-ACh3.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_ACh3.0
Plasmid#121923Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent mannerDepositorInsertGPCR activation based ACh sensor GRAB-ACh3.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIgkc
Plasmid#127157PurposeExpression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant regionDepositorInsertIgkc (Igkc Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIghg2b
Plasmid#127155PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2b isotypeDepositorInsertIghg2b (Ighg2b Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.1-mIglc2
Plasmid#127156PurposeExpression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), lambda 2 constant regionDepositorInsertIglc2 (Iglc2 Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTER GAMMA-TUBULIN shRNA Human
Plasmid#87955Purposereduced the expression of gamma-tubulin in human cellsDepositorInsertsh gamma-tubulin (TUBG1 Human)
UseTagsNo-tagExpressionMammalianMutationThe protein is a sh-gamma-tubulin resistant gene.…PromoterAvailable sinceOct. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-FOXA1-T2A-eGFP
Plasmid#182337PurposeExpresses human FOXA1 and eGFP via T2A linker under control of a CMV promoter.DepositorInsertForkhead Box A1 (FOXA1 Human)
UseTagsT2A-eGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV-FOXA1-T2A-miRFP670
Plasmid#182335PurposeExpresses human FOXA1 and miRFP670 via T2A linker under control of a CMV promoter.DepositorInsertForkhead Box A1 (FOXA1 Human)
UseTagsT2A-miRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-ccdB
Plasmid#166147PurposeGateway destination vector for generation of N-terminal His10-Smt3 tagged proteins in E. coli.DepositorArticleTypeEmpty backboneUseTagsHis10-Smt3ExpressionBacterialMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-DDX5(kf)-SNAP
Plasmid#166152PurposeExpression of N. furzeri DDX5 in E. coliDepositorArticleInsertDDX5
UseTagsHis10 and SNAPExpressionBacterialMutationPromoterT7Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG415GPD-EGFP
Plasmid#166137PurposeEGFP expression under the control of the GPD promoter yeastDepositorArticleInsertEGFP
UseTagsExpressionYeastMutationPromoterGDPAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG415GPD-mRuby3
Plasmid#166138PurposemRuby3 expression under control of the GPD promoter in yeastDepositorArticleInsertmRuby3
UseTagsExpressionYeastMutationPromoterGDPAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-EGFP
Plasmid#166140PurposeEGFP expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.DepositorArticleInsertEGFP
UseTagsExpressionYeastMutationPromoterGAL1Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-EGFP
Plasmid#166143PurposeEGFP expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.DepositorArticleInsertEGFP
UseTagsExpressionYeastMutationPromoterGAL1Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-mRuby3
Plasmid#166144PurposemRuby3 expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.DepositorArticleInsertmRuby3
UseTagsExpressionYeastMutationPromoterGAL1Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-ccdB-mRuby3
Plasmid#166139PurposeGateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a CEN/ARS element for low copy number when in yeast.DepositorArticleTypeEmpty backboneUseTagsmRuby3ExpressionYeastMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-FOXC1-T2A-miRFP670
Plasmid#182336PurposeExpresses human FOXC1 and miRFP670 via T2A linker under control of a CMV promoter.DepositorInsertForkhead Box C1 (FOXC1 Human)
UseTagsInserted T2A-miRFP670 at 3' terminal of MCS.ExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-mRuby3
Plasmid#166141PurposemRuby3 expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.DepositorArticleInsertmRuby3
UseTagsExpressionYeastMutationPromoterGAL1Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-ccdB-mRuby3
Plasmid#166142PurposeGateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a 2um element for high copy number when in yeast.DepositorArticleTypeEmpty backboneUseTagsmRuby3ExpressionYeastMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-ccdB-SNAP
Plasmid#166146PurposeGateway destination vector for generation of N-terminal His10-tagged and C-terminal SNAP-tagged proteins in E. coli.DepositorArticleTypeEmpty backboneUseTagsHis10 and SNAPExpressionBacterialMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-ccdB-SNAP
Plasmid#166148PurposeGateway destination vector for generation of N-terminal His10-Smt3 and C-terminal SNAP tagged proteins in E. coli.DepositorArticleTypeEmpty backboneUseTagsHis10-Smt3 and SNAPExpressionBacterialMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-DDX5(kf)-SNAP
Plasmid#166149PurposeExpression of N. furzeri DDX5 in E. coliDepositorArticleInsertDDX5
UseTagsHis10-Smt3 and SNAPExpressionBacterialMutationPromoterT7Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-DDX5ΔPrD(kf)-SNAP
Plasmid#166150PurposeExpression of N. furzeri DDX5ΔPrD(1-535) mutant in E. coliDepositorArticleInsertDDX5ΔPrD(1-535)
UseTagsHis10-Smt3 and SNAPExpressionBacterialMutationΔPrD(1-535)PromoterT7Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-DDX5ΔIDR(kf)-SNAP
Plasmid#166151PurposeExpression of N. furzeri DDX5ΔIDR(1-483) mutant in E. coliDepositorArticleInsertDDX5ΔIDR(1-483)
UseTagsHis10-Smt3 and SNAPExpressionBacterialMutationΔIDR(1-483)PromoterT7Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-SOX2-T2A-miRFP670
Plasmid#187375PurposeExpresses human SOX2 and miRFP670 via T2A linker under control of a CMV promoter.DepositorInsertSOX2 (SOX2 Human)
UseTagsT2A-miRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIFEX-A2aGH
Plasmid#160542PurposepITY4 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEFEX1-A2aGH
Plasmid#160543PurposepYES2 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEFEX2-A2aGH
Plasmid#160544PurposepYES2 derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEFEX3-A2aGH
Plasmid#160545PurposepYES2 derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFEX1-A2aGH
Plasmid#160546PurposepYC2/CT derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFEX2-A2aGH
Plasmid#160547PurposepYC2/CT derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFEX3-A2aGH
Plasmid#160548PurposepYC2/CT derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-RfxCas13d-HA
Plasmid#141320PurposePlasmid to carry out IVT of RfxCas13d (human codon-optimized)DepositorInsertRfx-Cas13d
UseCRISPRTagsHAExpressionMutationPromoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
SOX2-P2A-tagBFP-HDR
Plasmid#163751PurposeHDR knock-in template for tagging the human endogenous SOX2 gene with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.DepositorInsertSOX2 (SOX2 Human)
UseCRISPR and Synthetic BiologyTagsP2A-tagBFPExpressionMutationDoes not contain start codon to avoid random inte…PromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-RfxCas13d-His
Plasmid#141322PurposePlasmid for bacterial expression and purification of RfxCas13d proteinDepositorInsertRfx-Cas13d
UseCRISPRTags6xHisExpressionBacterialMutationPromoterT7Available sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only