Skip to main content
Addgene
Showing: 121 - 150 of 493 results

  1. USP5

    Plasmid
    #25299
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP5.001.835 (USP5 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. PP-UEV1A

    Plasmid
    #25111
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PFC0255c:C34858
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. PP-UBC13 (3E95)

    Plasmid
    #25131
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PFE1350c:C34878
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. sAB-K29

    Plasmid
    #204735
    Purpose
    synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin
    Depositor
    Minglei Zhao
    Article
    Yu et al Nat Chem Biol. 2021 Aug;17(8):896-905. d
    Insert
    synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    204735-plasmid-map-sequence-id-412652

    Enlarge

  9. p663-UBC-TurboID-V5-dsRed_IDG-K

    Plasmid
    #135261
    Purpose
    Gateway destination clone of dsRed (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TurboID-V5-dsRed
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135261-plasmid-map-sequence-id-268068

    Enlarge

  10. p667-UBC-GFP-V5-TurboID_IDG-K

    Plasmid
    #135241
    Purpose
    Gateway destination clone of GFP (as control) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    GFP-V5-TurboID
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135241-plasmid-map-sequence-id-268090

    Enlarge

  11. p663-UBC-miniTurbo-V5-eGFP_IDG-K

    Plasmid
    #135263
    Purpose
    Gateway destination clone of eGFP (as control) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-eGFP
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135263-plasmid-map-sequence-id-268067

    Enlarge

  12. p663-UBC-TurboID-V5-eGFP_IDG-K

    Plasmid
    #135264
    Purpose
    Gateway destination clone of eGFP (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TurboID-V5-eGFP
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135264-plasmid-map-sequence-id-268064

    Enlarge

  13. p667-UBC-GFP-V5-miniTurbo_IDG-K

    Plasmid
    #135240
    Purpose
    Gateway destination clone of GFP (as control) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    GFP-V5-miniTurbo
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135240-plasmid-map-sequence-id-268088

    Enlarge

  14. p667-UBC-hcRed-V5-miniTurbo_IDG-K

    Plasmid
    #135237
    Purpose
    Gateway destination clone of hcRed (as control) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    hcRed-V5-miniTurbo
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135237-plasmid-map-sequence-id-311572

    Enlarge

  15. p663-UBC-V5-BioID-STOP_IDG-K

    Plasmid
    #158163
    Purpose
    Gateway destination clone of BioID (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-BioID
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    158163-plasmid-map-sequence-id-328021

    Enlarge

  16. DTX3L

    Plasmid
    #28266
    Depositor
    Cheryl Arrowsmith
    Insert
    DTX3L (DTX3L Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    contains only carboxyl terminal domain (residues …
    Promoter
    Available since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    28266-plasmid-map-sequence-id-463306

    Enlarge

  17. p667-UBC-hcRed-V5-TurboID_IDG-K

    Plasmid
    #135238
    Purpose
    Gateway destination clone of hcRed (as control) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    hcRed-V5-TurboID
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    135238-plasmid-map-sequence-id-268556

    Enlarge

  18. p663-UBC-miniTurbo-V5-dsRed_IDG-K

    Plasmid
    #135260
    Purpose
    Gateway destination clone of dsRed (as control) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-dsRed
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    135260-plasmid-map-sequence-id-311150

    Enlarge

  19. phage - ubc - nls - 2xmcp - egfp - cMycBirA*

    Plasmid
    #131133
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Insert
    NLS-HA-2xMCP-EGFP-cMycBirA*
    Use
    Lentiviral
    Tags
    NLS, HA, cMyc (N-terminal of BirA*)
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131133-plasmid-map-sequence-id-255501

    Enlarge

  20. USP21 (3I3T)

    Plasmid
    #25147
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    usp21.209.563 (USP21 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  21. USP15

    Plasmid
    #25301
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    usp15.0001.0133 (USP15 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. UBE2G1

    Plasmid
    #25614
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    UBE2G1 (UBE2G1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 31, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  23. UBC46

    Plasmid
    #25470
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubc46.055.201 (UBE2E2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. UBH26

    Plasmid
    #25467
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubh26.019.099 (UBQLN3 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids 19-99
    Promoter
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. UBC81

    Plasmid
    #25218
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubc81.171.471 (UEVLD Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. pAAV-i53

    Plasmid
    #92170
    Purpose
    recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1
    Depositor
    Daniel Durocher
    Article
    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d
    Insert
    i53
    Use
    AAV
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    UbvG08 with I44A mutation and no terminal Glycines
    Promoter
    CMV
    Available since
    July 13, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    92170-plasmid-map-sequence-id-182617

    Enlarge

  27. pcDNA3.1-N-Myc_DED/AAA 535-888 USP7

    Plasmid
    #131255
    Purpose
    Mammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    A mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131255-plasmid-map-sequence-id-267709

    Enlarge

Showing: 121 - 150 of 493 results