-
Plasmid#84927Purpose–2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vectorDepositorInsert–2.3kb promoter of zebrafish fabp2/ifabp gene (fabp2 Zebrafish)
UseTagsNOExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2+cPLA2-mKate2
Plasmid#162068PurposeUsed to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseAvian, xenopus, zebrafishTagsmKate2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2-elavl3-H2B-GCaMP6s
Plasmid#59530PurposeExpressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsUseTransposable elementTagsExpressionMutationPromoterAvailable sinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
zCD2
Plasmid#122031PurposeExpresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.DepositorInsertZebrafish HDAC6 Catalytic Domain 2
UseTagsHis6-MBP tag with cleavable TEV siteExpressionBacterialMutationPromoterT7Available sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro dr_cPLA2-mKate2
Plasmid#163844PurposeUsed as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseTagsmKate2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5E-mfap4
Plasmid#70052Purpose5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.DepositorInsertmfap4 promoter
UseGateway 5' elementTagsExpressionMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGTag-eGFP-SV40
Plasmid#117813PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInserteGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Danio alpha A-crystallin 1kb promoter/AcGFP
Plasmid#98096PurposeaA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryosDepositorInsertAlpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)
UseGfp expressingTagsGFPExpressionMutationPromoterDanio alpha A-crystallin 1 kb fragment (-1028/-1)Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKHR8
Plasmid#74625Purposea plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.DepositorInsertsFRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
cmlc2::mCherry
UseCre/Lox; Zebrafish expressionTagsExpressionMutationPromoteralpha-crystallin and cmlc2Available sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3E_he1a:mCherry
Plasmid#113881Purposep3E entry vector for Three Multisite GatewayDepositorInserthe1a (he1.1 Zebrafish)
UseZebrafish danio expressionTagsmCherryExpressionMutationPromoterAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Zf GFP-vclb
Plasmid#185411PurposeGFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMVDepositorInsertvinculin b (vclb Zebrafish)
UseIn vitro synthesis of mrnaTagseGFPExpressionMammalianMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSYC-437
Plasmid#163829PurposeFoxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.DepositorInsertsFoxj1a Promoter
GCaMP6s
UseMultisite gateway destination vectorTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGTag-gal4-VP16-B-actin
Plasmid#117817PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertgal4/VP16
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Zf GFP-vcla
Plasmid#185410PurposeGFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMVDepositorInsertvinculin a (vcla Zebrafish)
UseIn vitro synthesis of mrnaTagseGFPExpressionMammalianMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCK069 desmaMCS:minprom_mApple,exorh:EGFP
Plasmid#195982PurposeExpression constructDepositorInsertdesmin a regulatory elements, mouse beta-globin minimal promoter mApple, exorh:EGFP (desma Zebrafish)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
acta1-Cavin4b-Clover
Plasmid#163854PurposeZebrafish muscle specific expression of Cavin4b-Clover. Parton lab clone DQZDepositorInsertCavin4b (cavin4b Zebrafish)
UseZebrafish expressionTagsCloverExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXTm-PH domain
Plasmid#210326PurposeExpresses EGFP with PH domainDepositorInsertPH domain
UseFish expressionTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pminiTol2-4kb Wnt3 promoter-EGFP
Plasmid#203792PurposeTol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of EGFP for zebrafish transgenesisDepositorInsertEGFP
UseZebrafish expressionTagsExpressionMutationPromoter4kb Wnt3 promoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus
Plasmid#105127PurposeUsed along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent proteinDepositorInsertsUseZebrafish transgenesisTagsExpressionMutationPromotercryaa (crystallin) and fabp10aAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
punc503GFP
Plasmid#64021Purpose503-bp promoter of zebrafish unc-45b drives muscle-specific GFP expressionDepositorInsertunc-45b: GFP (unc45b Zebrafish)
UseGatewayTagsExpressionMutationPromoter503-bp promoter of zebrafish unc-45bAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only