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Plasmid#188007PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker insulin BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; insulin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NI_G1333-DddA11-N
Plasmid#191609PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NN_G1333-DddA11-N
Plasmid#191610PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_HD_G1333-DddA11-C
Plasmid#191599PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NG_G1333-DddA11-C
Plasmid#191600PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NI_G1333-DddA11-C
Plasmid#191601PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NN_G1333-DddA11-C
Plasmid#191602PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_HD_G1333-DddA11-N
Plasmid#191607PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NG_G1333-DddA11-N
Plasmid#191608PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCT03
Plasmid#212150PurposePlasmid expressing alanine dehydrogenase under inducible promoter PT7 control. The enzyme is thermophilic and works best at 50 C.DepositorInsertalanine dehydrogenase
UseTags6x HisExpressionBacterialMutationPromoterPT7Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmVen-C17orf53
Plasmid#211419Purposemammalian expression of human C17orf53 N-terminally fused to mVenusDepositorInsertC17orf53 (HROB Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCer-C17orf53
Plasmid#211418Purposemammalian expression of human C17orf53 N-terminally fused to mCeruleanDepositorInsertC17orf53 (HROB Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmVen-Mcm5
Plasmid#211392Purposemammalian expression of human Mcm5 N-terminally fused to mVenusDepositorInsertMcm5 (MCM5 Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVen-RPA32-IRES-RPA14
Plasmid#211429Purposemammalian expression of human RPA32 N-terminally fused to mVenus and RPA14DepositorUseTagsmVenus (on RPA32)ExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_CHLP8
Plasmid#204242PurposeBacterial expression of C. parvum (green sulfur photosynthetic bacterium) malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_CHLP8
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAPN2A-c002
Plasmid#205104PurposeCAPN2 protein expressionDepositorInsertCAPN2 (CAPN2 Human)
UseTags6His-TEV and AviTagExpressionBacterialMutationWTPromoterAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mOct4 Full length(FL)
Plasmid#206394PurposeExpresses Oct4 with a 6xHis tag for protein purificationDepositorInsertOct4 (Pou5f1 Mouse)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR3cDual-Fray
Plasmid#203977PurposeEnrty clone for Gateway cloningDepositorInsertFrayed (fray Fly)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
eDrTrkB
Plasmid#183997PurposeOptically controlled eDrTrkB expression in mammalian cellsDepositorInserteDrTrkB (NTRK2 Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Ex1-17-deltaP1P2-PTPN22
Plasmid#195391PurposeMammalian expression of a human PTPN22 mutant (exon 1-17 without the P1 and P2 domains) with FLAG tagDepositorInsertPTPN22 (PTPN22 Human)
UseTagsFLAGExpressionMammalianMutationPTPN22 mutant containing exon 1 to 17 and lacking…PromoterAvailable sinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only