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Plasmid#99893PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only
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pG4-CMV-luc
Plasmid#46322DepositorInsert5xGal4
UseLuciferaseTagsCMV promoter and luciferaseExpressionMammalianMutationfive copies of a multimerized GAL4 (5×GAL4) site …PromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS-puro shNEDD4L #2
Plasmid#27017DepositorInsertshRNA NEDD4L #2 (NEDD4L Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131C2.0
Plasmid#99886PurposeGolden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU6Available sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132C2.0
Plasmid#99889PurposeGolden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS-puro shNEDD4L #1
Plasmid#27016DepositorInsertshRNA NEDD4L #1 (NEDD4L Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAT9752-BEAR-mScarlet
Plasmid#162991PurposeBEAR target plasmid with split mScarlet and disrupted 5' splice siteDepositorInsertmScarlet split with an intron between amino acids 110-111
UseCRISPRTagsExpressionMammalianMutationdisrupted 5' splice sitePromoterCMVAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati
Plasmid#91800PurposeExpresses the Drosophila dati circular RNADepositorInsertdati (dati Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
EF1a_TBX5_P2A_Hygro_Barcode
Plasmid#120486PurposeBarcoded lentiviral vector to express TBX5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertTBX5 (TBX5 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGM202T7
Plasmid#69993PurposeE. coli/Streptomyces expression vector containing the T7-promoterDepositorInsertsaphII
tsr
rep
sso
T7 promoter
dso
His-Orf
UseTagsN-terminal and C-terminal His tag encoding sequen…ExpressionBacterialMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only