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Plasmid#167158PurposeGolden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only
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p5E-4xnrUAS
Plasmid#87120PurposeMultisite gateway entry clone containing 4 non-repetitive UAS (upstream activation sequence) elementsDepositorInsert4xnrUAS (non repetitive)
UseGateway vectorTagsExpressionMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
DDX3X in pENTR
Plasmid#216572Purposegateway cloning donor vector containing DDX3XDepositorInsertDDX3X (DDX3X Human)
UseGateway entry plasmidTagsExpressionMutationPromoterAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDropVn
Plasmid#184873PurposeChromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.DepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYPQ143-ZmUbi-tRNA
Plasmid#158400PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ133-tRNA2.0; assembly of 3 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-Fv2E-flt4um200-ns
Plasmid#67696Purposemiddle entry plasmid with dimerizable extracellular domain fused to um200 mutant Danio Flt4 cytoplasmic domainDepositorInsertFv2E-flt4um200
UseTagsHAExpressionMutationum200, no stop codon; N1056SPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pV1093
Plasmid#111428PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p15A_SpyCas9_CmR
Plasmid#119162PurposeExpresses S. pyogenes Cas9DepositorInsertCas9 from S. pyogenes (NEWENTRY )
UseCrisprTagsExpressionMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ142-ZmUbi-tRNA
Plasmid#158578PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 and pYPQ132-tRNA2.0; assembly of 2 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
C125-E01: stuffer
Plasmid#162900PurposeStandard Gateway entry clone containing a non-transcribed/translated region for use in generating negative control vectorsDepositorInsertstuffer fragment for vector controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only