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MELK gRNA (BRDN0001145853)

Plasmid

#75668

Purpose

3rd generation lentiviral gRNA plasmid targeting human MELK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MELK (MELK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLX302-PTP-SL-V5 puro

Plasmid

#87774

Purpose

Expresses V5 tagged human PTP-SL

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

PTP-SL (PTPRR Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-OCT4

Plasmid

#35391

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE-Oct4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2012

Availability

Academic Institutions and Nonprofits only
pSicoR-Ef1a-mCh-GFPi

Plasmid

#31849

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

GFP RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6 for shRNA; Ef1alpha for mCherry

Available since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-mNG-eDHFR(69K6)-RasGEF

Plasmid

#172104

Purpose

Mammalian expression of RasGRF1(Cdc25) fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-RasGRF1(Cdc25) (RASGRF1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSico Spry-2

Plasmid

#14809

Depositor

Tyler Jacks

Article

Shaw et al Genes Dev. 2007 Mar 15. 21(6):694-707.

Insert

Sprouty-2 shRNA (Spry2 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
FgH1tUTG_hup53_Ex4

Plasmid

#85538

Purpose

Inducible expression of guide RNA (hup53_Ex4) with fluorescent GFP reporter

Depositor

Marco Herold

Insert

hup53 Ex4

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

H1t

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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sgRNA1_NANOG

Plasmid

#64153

Purpose

Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_NANOG (NANOG Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLX304-PCDH7-c

Plasmid

#108540

Purpose

Expresses human PCDH7 transcript variant c in mammalian cells

Depositor

Kathryn O’Donnell

Article

Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

Insert

PCDH7 transcript variant c (PCDH7 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304-PCDH7-b

Plasmid

#108539

Purpose

Expresses human PCDH7 transcript variant b in mammalian cells

Depositor

Kathryn O’Donnell

Article

Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

Insert

PCDH7 transcript variant b (PCDH7 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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PLKO.1-REG1-CDS

Plasmid

#136053

Purpose

REG1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (TATGGGATCAAGTGCCGATTC)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

REG1 (ZC3H12A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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GSK3A gRNA (BRDN0001147712)

Plasmid

#76372

Purpose

3rd generation lentiviral gRNA plasmid targeting human GSK3A

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GSK3A (GSK3A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-LITAF

Plasmid

#178444

Purpose

doxycycline-inducible expression of Human LITAF in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

LITAF (LITAF Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pWPI-TFDP1

Plasmid

#114299

Purpose

Constitutive expression of the TFDP1 protein

Depositor

Patrick Salmon

Article

Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

Insert

TFDP1 (TFDP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/V5-DEST-PKCi-A120E-ΔPB1

Plasmid

#56658

Purpose

expresses constitutively active human PKCi with a deletion on the PB1 domain

Depositor

Pedro Salas

Article

Forteza et al Biol Open. 2013 Oct 8;2(11):1264-9. doi:

Insert

Human PKCi (PRKCI Human)

Use

Lentiviral

Tags

V5/His

Expression

Mutation

A120E and deletion of PB1 domain (aa 24–98)

Promoter

CMV

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only
PPP2R2A B5.5 gRNA

Plasmid

#90847

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP2R2A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP2R2A (Guide Designation B5.5)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 E144K

Plasmid

#167193

Purpose

puromycin resistant lentiviral vector for expression of PAK5 E144K

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 E144K

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

UBC

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 M173I

Plasmid

#167192

Purpose

puromycin resistant lentiviral vector for expression of PAK5 M173I

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 M173I

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

UBC

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTCRa_candidate10

Plasmid

#165007

Purpose

Expression of candidate TCR #10 TCRalpha cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry_LacZ_sgRNA

Plasmid

#155108

Purpose

lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting LacZ

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

LacZ_sgRNA_2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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