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pAAV-CaMKIIa-hChR2(E123A)-mCherry

Plasmid

#35506

Purpose

AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Has Service

AAV9

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123A

Promoter

CaMKIIa

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pET28-MHL: nsp16_SARS-CoV-2

Plasmid

#167850

Purpose

Bacterial expression of the SARS-CoV-2 protein nsp16

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp16

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 16, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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C terminal Flag Mios pRK5

Plasmid

#46326

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Mios (MIOS Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(E123A)-EYFP

Plasmid

#35505

Purpose

AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Has Service

AAV9

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123A

Promoter

CaMKIIa

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pFL His-mEos3.2-I3

Plasmid

#113516

Purpose

Expression of His-Eos-I3 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

I3 (PPP1R11 Human)

Use

Tags

6*His-mEos3.2

Expression

Insect

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9

Plasmid

#84261

Purpose

Expresses AND gate ABA/GA-inducible VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

GAI-tagBFP-Sp dCas9
PYL1-VPR-p2a-GID1-ABI

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, GAI, GID1, HA tag, PYL1, p2a, …

Expression

Mammalian

Mutation

Promoter

CAG and PGK

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF10-2xCSTREP

Plasmid

#136174

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF10

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF10

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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FH1-MGT#1-mVenus

Plasmid

#164094

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

MGT#1-mVenus

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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ETV4_pet28a

Plasmid

#131645

Purpose

Bacterial expression of ETS gene ETV4

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV4 (ETV4 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-4591NES

Plasmid

#138377

Purpose

Booster-ROCK, a Red-Shifted Genetically Encoded FRET Biosensor for ROCK activity.

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Booster-ROCK

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Cep123 (CCDC123/Cep89)

Plasmid

#67787

Purpose

This plasmid encodes EGFP fused to the N-terminus of isoform 1 of Cep123 (CCDC123/Cep89)

Depositor

Michel Bornens

Article

Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

Insert

Cep123 (CEP89 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-GT

Plasmid

#26198

Depositor

Edward Callaway

Article

Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Enhanced green fluorescent protein, TVA (eGFP TVA is a mouse codon optimized version of an avian gene., Mouse)

Use

AAV and Cre/Lox

Tags

Expression

Mutation

TVA mammalian codon optimized

Promoter

Available since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123A)-EYFP

Plasmid

#35507

Purpose

AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123A

Promoter

Ef1a

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HLA-cmyc-natT1R2

Plasmid

#113946

Purpose

mammalian expression plasmid for c-myc-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Use

Tags

Signal/leader sequence from HLA class I histocomp…

Expression

Mammalian

Mutation

residues 22-839

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-1delta

Plasmid

#124142

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Tags

Expression

Mutation

None (Wild Type)

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBig1a zz TEV YBBR RAP1 ZZ TEV TRF2

Plasmid

#185449

Purpose

Coexpresses human RAP1 with an MBP affinity tag, YBBR site, and TEV site, and human TRF2 with a zz tag and TEV site

Depositor

Ahmet Yildiz

Article

Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

Inserts

TRF2 (TERF2 Human)
RAP1 (TERF2IP Human)

Use

Tags

MBP, YBBR, and ZZ

Expression

Insect

Mutation

Promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA-Ago2-Y529E

Plasmid

#215842

Purpose

Expression of AGO2-Y529E mutant

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

Human AGO2 (AGO2 Human)

Use

Tags

Expression

Mammalian

Mutation

Ago2-Y529E

Promoter

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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IMPT-2070

Plasmid

#172092

Purpose

pFastBac1 vector with GP64 promoter replacing polH promoter expressing A2aAR+BRIL GPCR

Depositor

Raymond Stevens

Article

Liu et al Science. 2012 Jul 13;337(6091):232-6. do

Insert

Homo sapiens adenosine A2a receptor (ADORA2A) (ADORA2A Human)

Use

Tags

cytochrome b562RIL

Expression

Insect

Mutation

Promoter

gp64

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (BTB+BACK)

Plasmid

#169723

Purpose

Mammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, I217K, Y353E)

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

L186D, L190D, L193D, I217K, Y353E

Promoter

CMV

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK-Hygro-Myc-hAgo2

Plasmid

#60234

Purpose

Express HA-tagged wild-type Ago2 in mammalian cells in a Dox-inducible manner

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2012 May 25;46(4):507-17. doi:

Insert

Argonaute2 (AGO2 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2014

Availability

Academic Institutions and Nonprofits only