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Plasmid#186036PurposeAAV-mediated cytosolic expression of jGCaMP8m under the synapsin promoterDepositorInsertNES-jGCaMP8m
UseAAVTagsNES-6xHisExpressionMammalianMutationPromotersynapsinAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG35
Plasmid#35123DepositorInsertnatMX3
UseYeast replicatingTagsExpressionMutationPromoterAvailable sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-NES-jGCaMP8f-WPRE
Plasmid#186035PurposeAAV-mediated cytosolic expression of jGCaMP8f under the synapsin promoterDepositorInsertNES-jGCaMP8f
UseAAVTagsNES-6xHisExpressionMammalianMutationPromotersynapsinAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIG3 Rab5CA
Plasmid#78268PurposeRab5 Constitutively active (Q79L) expressionDepositorInsertRAB5A (RAB5A Human)
UseLentiviralTagsIRES-EGFP and mCherryExpressionMammalianMutationQ79LPromoterCMV, Lentiviral LTRAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG36
Plasmid#35126DepositorInsertnat1
UseYeast replicatingTagsExpressionMutationPromoterAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAG34
Plasmid#35125DepositorInserthphMX4
UseYeast replicatingTagsExpressionMutationPromoterAvailable sinceSept. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
p(+)MV-NSE-FlagrP-3g_haP-F497A-3p
Plasmid#58799PurposeExpresses recombinant measles virus with duplicated P gene. wt P gene and P-F497A gene are tagged with Flag and HA peptides and si1 (siGFP) and si2 (siP) target sequences, respectively.DepositorInsertMeasles virus antigenome
UseTagsFlag and HaExpressionMutationPromoterT7Available sinceAug. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
dClover2-Cx43-7
Plasmid#56532PurposeLocalization: Gap Junctions, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.DepositorInsertCx43
UseTagsdClover2ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
p(+)MV-NSE-FlagrP-3g_haP-M502-3p
Plasmid#58798PurposeExpresses recombinant measles virus with duplicated P gene tagged with Flag and HA peptides and si1 (siGFP) and si2 (siP) target sequences, respectively.DepositorInsertMeasles virus antigenome
UseTagsFlag and HaExpressionMutationPromoterT7Available sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
p(+)MV-NSE-FlagrP-3g_haP-F497D-3p
Plasmid#58800PurposeExpresses recombinant measles virus with duplicated P gene. wt P gene and P-F497D gene are tagged with Flag and HA peptides and si1 (siGFP) and si2 (siP) target sequences, respectively.DepositorInsertMeasles virus antigenome
UseTagsFlag and HaExpressionMutationPromoterT7Available sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-N
Plasmid#218398Purpose3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_N-H1
Plasmid#218397Purpose3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB4A3-PBAD-phzSM
Plasmid#165620PurposeArabinose-inducible expression of phzSM for conversion of phenazine-1-carboxylic acid to pyocyaninDepositorUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXR003_puroR
Plasmid#219819PurposehU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOpen-phi29pol
Plasmid#165573PurposeDNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.DepositorInsertphi29 DNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pECNUS4
Plasmid#184887PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRPS5AAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG26
Plasmid#35127DepositorInserthph
UseYeast replicatingTagsExpressionMutationPromoterAvailable sinceAug. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAG61
Plasmid#35129DepositorInsertURA3
UseYeast replicatingTagsExpressionMutationPromoterAvailable sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pActP-GCaMP6m
Plasmid#64714Purposevisualizing calcium in cultured Drosophila S2 cellsDepositorInsertGCaMP6m
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22B_PcrA_2Bchimera
Plasmid#102998PurposeExpress a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrADepositorInsertPcrA_2Bchimera
UseTagsExpressionBacterialMutationBacilllus stearothermophilus PcrA in which the 2B…PromoterT7Available sinceMay 24, 2018AvailabilityAcademic Institutions and Nonprofits only