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Plasmid#103131PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertQ927P4(Cas9 coding gene from Listeria innocua serovar 6a (strain CLIP 11262))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1B POLE1_2
Plasmid#160765PurposeSuppress POLE1DepositorInsertshPOLE1_2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1B POLE1_1
Plasmid#160763PurposeSuppress POLE1DepositorInsertshPOLE1_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1
Plasmid#192471PurposeTransient expression of RvCAHS12 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cellsDepositorInsertCAHS12
UseTagsAcGFP1ExpressionInsectMutationPromoterAc5Available sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI
Plasmid#106895PurposeExpresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulationDepositorInsertsGAI-tagBFP-Sp dCas9-ABI
GID1-KRAB
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …ExpressionMammalianMutationPromoterCAG and PGKAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-DR-GC-swap-cr in pBCSK+
Plasmid#126643PurposeCloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cellsDepositorInsertLbCas12a Full Length Direct Repeat GC Swap crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMP71-mtGFP-llo-lama4-alg8
Plasmid#174605Purposeexpresses palmytoylated GFP with a C terminal fusion of the LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 and Lama4DepositorInsertpalmytoylGFP w/C-term fusion LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 Lama4
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
His6tev CBP BCHZDeltaAL
Plasmid#99551PurposeBacterial expression of His tagged mouse CBP HAT Brd-ZZ(1079-1751) 1557-1617 replaced by SGGSGDepositorInsertmouse CBP BRD-ZZ domains (1079-1751) DeltaAL (Crebbp Mouse)
UseTagsHis6tevExpressionBacterialMutationreplaced 1557-1618 with SGGSGPromoterT7Available sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Designed VEGF-binding scFv G6des13
Plasmid#110213PurposeYeast Surface Display of G6des13DepositorInsertG6des13
UseTagscmyc tagExpressionYeastMutationL A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95FPromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_Rat_cebp-alfa
Plasmid#140098PurposeCRISPR-Cas9 library validation (positive control)DepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_SPI1
Plasmid#140099PurposeCRISPR-Cas9 library validation (positive control)DepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
YCplac33-NAT_MCART1
Plasmid#140591PurposeExpress MCART1 in S. cerevisiaeDepositorInsertSLC25A51 with 5' and 3' UTR of scNDT1 (SLC25A51 Human)
UseTagsExpressionYeastMutationcodon-optimized for expression in S. cerevisiaePromoterNDT1 promoter and 3'UTRAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIG AKT3 S472E
Plasmid#73053PurposeHuman AKT3 S472EDepositorInsertAKT3 S472E (AKT3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB65 - pL0_tGFP-I (CDS1)
Plasmid#123182PurposeGolden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)DepositorInsertturboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
UsePart for plant expressionTagsExpressionPlantMutationNo BpiI and BsaI sites; codon-optimised for plantsPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS0035
Plasmid#131125PurposeMammalian Target-AID expression vectorDepositorInsertnCas9-PmCDA1-UGI
UseCRISPRTagsPmCDA1 and UGIExpressionMammalianMutationD10A in SpCas9PromoterCMV promoterAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ai39
Plasmid#34884DepositorInsertCAG-Floxed eNpHRv3.0-EYFP
UseMouse TargetingTagsEYFPExpressionMutationPromoterCAGAvailable sinceFeb. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSW554 - pOp5:SP-mVenus-HDEL
Plasmid#116006Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpOp5:SP(ER)-mVenus-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nanog-luciferase targeting construct
Plasmid#26591DepositorInsertNanog promoter-luciferase-lox-pgk-neomycin-loc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pQCascade-IS3
Plasmid#140626PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS3. The crRNA-IS3 targets IS3 loci in Escherchia coli.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS3
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-AS9 (Mito MTS)-Delta N67-mMCL1
Plasmid#45822DepositorInsertAS9(Mito MTS)-Delta N67-mMCL1
UseTagsExpressionMammalianMutationATP Synthase subunit 9 is used as a Mito Targetin…PromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only