pSW554 - pOp5:SP-mVenus-HDEL
(Plasmid
#116006)
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Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 116006 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneZ001
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Vector typePlant Expression
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Selectable markersSulfadiazine, mVenus
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepOp5:SP(ER)-mVenus-HDEL:tUBQ10::SulfR
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)3438
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Tags
/ Fusion Proteins
- SP(ER) (N terminal on insert)
- HDEL (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSW554 - pOp5:SP-mVenus-HDEL was a gift from Sebastien Wolf (Addgene plasmid # 116006 ; http://n2t.net/addgene:116006 ; RRID:Addgene_116006) -
For your References section:
A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene Expression in Arabidopsis. Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb T, Wolf S. Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463. 10.1104/pp.18.00463 PubMed 30026289
