Addgene

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p5E_ngn1_LSE_C

Plasmid

#199395

Purpose

pDEL98; Gateway 5' Entry clone encoding select ngn1 upstream regulatory sequence

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

LSE_C

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pYTK001_E2Aq(1st)

Plasmid

#183088

Purpose

2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

E2A

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2Aq(2nd)

Plasmid

#183089

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1_Violet

Plasmid

#183139

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_Violet

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E_Violet

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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C122-E15: mOrange

Plasmid

#162895

Purpose

Standard Gateway entry clone encoding mOrange fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Orange fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C122-E14: mKate

Plasmid

#162894

Purpose

Standard Gateway entry clone encoding mKate fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Kate fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C122-E10: mCherry

Plasmid

#162892

Purpose

Standard Gateway entry clone encoding mCherry fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Cherry fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C121-E06: gLuc

Plasmid

#162890

Purpose

Standard Gateway entry clone encoding Gaussia luciferase

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Gaussia princeps luciferase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C121-E01: rLuc

Plasmid

#162888

Purpose

Standard Gateway entry clone encoding Renilla luciferase

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Renilla luciferase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C122-E54: mRuby3

Plasmid

#162899

Purpose

Standard Gateway entry clone encoding mRuby3 fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Ruby3 fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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