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Plasmid#109139PurposeGFP-tagged CYFIP1 expression constructDepositorInsertCYFIP1 (CYFIP1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCiGSΔΨ
Plasmid#108312PurposeFoamy virus helper plasmid expressing Gag, driven by a CMV promoter. Ampicillin resistant.DepositorInsertgag
UseTagsExpressionMammalianMutationpCiGSΔΨ is a newer version of the pCiGS Gag expre…PromoterCMVAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv1(mCherry-FLAG) in pTwist-CMV
Plasmid#216165PurposeExpression of mCherry specifically in cells with TDP-43 knockdown. Uses AARS1-derived cryptic exon to control mCherry expression.DepositorInsertmCherry with C-terminal FLAG tag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-MVP
Plasmid#204543PurposeExpresses EGFP-tagged MVP in mammalian cellsDepositorInsertMVP (MVP Human)
UseTagsExpressionMammalianMutationNonePromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-ORF52-2xCSTREP
Plasmid#136212PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF52DepositorInsertORF52
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub(TA)-YPet-NES
Plasmid#84636PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub(TA)-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
FSW-HRP-V5-LRRTM2
Plasmid#82537PurposeLentiviral vector for expressing HRP-LRRTM2 in neuronsDepositorInsertLRRTM2 (LRRTM2 Human)
UseLentiviralTagsHRP and V5ExpressionMammalianMutationPromoterHuman SynapsinAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
EGFP-C1-FKBP-CYB5tail
Plasmid#202686Purposerapamycin-induced recruitment of proteins to ERDepositorInsertEGFP:FKBP:[GGSA]4GG:CYB5A(100-134) (CYB5A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV CytoBAS
Plasmid#62860PurposeFluorescent bile acid sensor (BAS) that allows spatiotemporal monitoring of bile acid transport in single living cells using FRETDepositorInsertCytoBAS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFB2B
Plasmid#195284PurposeBicistronic HSV-TK and Puromycin resistance for positive/negative selectionDepositorInsertHSV-TK 2A Puro
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-COV M
Plasmid#165124Purposemammalian expression and localizationDepositorInsertCOV M (M codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp12
Plasmid#157709Purposemammalian expression of untagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoterDepositorInsertNsp12 (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-Nsp8-HF
Plasmid#157696Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoterDepositorInsertNsp8-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-Nsp8
Plasmid#157694Purposemammalian expression of untagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoterDepositorInsertNsp8 (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL3-E-cadherin promoter
Plasmid#61798PurposeLuciferase reporter containing the mouse E-cadherin promoterDepositorInsert-178/+92 fragment of the mouse Cdh1 promoter (Cdh1 Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFP-COV E
Plasmid#165123Purposemammalian expression and localizationDepositorInsertCOV E (E codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Cep131
Plasmid#136826PurposeMammalian expression of the centrosomal protein Cep131 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep131 (CEP131 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-ORF50-2xCSTREP
Plasmid#136211PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF50DepositorInsertORF50
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3
Plasmid#127353Purposegateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteinsDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and mVenusExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-3-SARS-CoV-2-3CL
Plasmid#168457PurposeBacterial codon optimized expression vector for SARS-CoV-2 3CL protease.DepositorInsertSARS-CoV-2 3CL protease (ORF1ab Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMarch 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits