Addgene

We narrowed to 34,843 results for: lat

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p18S.7 tag

Plasmid

#51730

Purpose

transcribes truncated transcript containing 18S and hybridization tag

Depositor

Vincent Mauro

Article

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

18S

Use

Tags

Expression

Mutation

Promoter

pol-I

Available since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pCMVhPOP1_3Flag

Plasmid

#53968

Purpose

Mammalian expression vector for human POP1 with c-terminal 3xFLAG epitopes.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

POP1 (POP1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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Integrin-β6 Headpiece I270C

Plasmid

#159638

Purpose

Contains integrin beta 6 residues 1–474 with I270C mutation

Depositor

Timothy Springer

Article

Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

Insert

Integrin beta 6 headpiece I270C

Use

Tags

6x His

Expression

Mammalian

Mutation

I270C

Promoter

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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RAB14 WT

Plasmid

#61493

Purpose

to overexpress wild type RAB14. Vector includes GFP driven by hUbC promoter

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

RAB14 (RAB14 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
pLL3.7 Scr shRNA

Plasmid

#59299

Purpose

non-targeting shRNA

Depositor

Ulrike Heberlein

Article

Lasek et al Genes Brain Behav. 2007 Nov;6(8):728-35.

Insert

Scr shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only
stathmin-GFP

Plasmid

#86782

Purpose

Mammalian expression of stathmin-GFP

Depositor

Lynne Cassimeris

Article

Ringhoff et al Mol Biol Cell. 2009 Aug;20(15):3451-8. d

Insert

stathmin (STMN1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-MinE-IRES-Blast

Plasmid

#213115

Purpose

Expression of untagged MinE in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

MinE (minE )

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSFVCs-lacZ

Plasmid

#92076

Purpose

This is a high level expression plasmid for production of recombinant genomic SFV RNA encoding a C-beta galactosidase fusion protein.

Depositor

Henrik Garoff

Article

Sjoberg et al Biotechnology (N Y). 1994 Nov;12(11):112

Insert

C-lacZ

Use

C-lacz expression plasmid, for run-off transcript…

Tags

Expression

Mutation

Promoter

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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p5193 pGL4-LCR-HPV16-LCR (nt 7000-100)-luciferase

Plasmid

#32888

Depositor

Peter Howley

Article

Ottinger et al Virology. 2009 Dec 20;395(2):161-71.

Insert

LCR HPV16

Use

Tags

Expression

Mutation

Promoter

LCR

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
ROPY (sYFP2-OP18-mScI-R125I)

Plasmid

#128002

Purpose

Improved redshifted FRET-readout of Stathmin/OP18 interaction with α-tubulin

Depositor

Dorus Gadella

Article

Van Geel et al Sci Rep. 2020 Apr 7;10(1):6034. doi: 10.

Insert

sYFP2-OP18-mScI-R125I

Use

Tags

Expression

Mammalian

Mutation

R125I in mScarlet-I

Promoter

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TRE_GFP

Plasmid

#25767

Purpose

Entry vector with TRE promoter driving GFP expression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

GFP

Expression

Mutation

Promoter

Available since

July 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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RNT2-GFP

Plasmid

#17709

Depositor

Hal Dietz

Article

Mendell et al Mol Cell Biol. 2000 Dec . 20(23):8944-57

Insert

rent2 (UPF2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pETZZtevPP7CP(C68A,C73A)

Plasmid

#27548

Depositor

Kathleen Collins

Article

Hogg et al RNA. 2007 Jun . 13(6):868-80.

Insert

Pseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)

Use

Tags

TEV protease cleavage site, six histidine tag, an…

Expression

Bacterial

Mutation

changed two cysteine to alanine (C68A,C73A) to in…

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pISH-Th

Plasmid

#105988

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Walz et al J Neurobiol. 2006 Jul . 66(8):835-46.

Insert

tyrosine hydroxylase (Th Mouse)

Use

To generate riboprobe

Tags

Expression

Mutation

Promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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RSV-betaGal

Plasmid

#24058

Depositor

Herbert Samuels, Frederick Stanley

Article

Flug et al J Biol Chem. 1987 May 5. 262(13):6373-82

Insert

LacZ (lacZ E. coli)

Use

Tags

RSV promoter

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
TOPO GFP

Plasmid

#68488

Purpose

TOPO construct expressing GFP for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Green Fluorescent Protein

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAFZifPZ431

Plasmid

#185849

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>ZifPZ43>MED3>PABF1

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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RAP1A

Plasmid

#55664

Purpose

expression of mouse RAP1A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RAP1A (Rap1a Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pJAT75

Plasmid

#204306

Purpose

Flippase under heat shock control. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

ie1-EGFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

ie1

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pKL13-MgtC bb118

Plasmid

#158982

Purpose

Constitutive expression (sigma 70 promoter) of mgtC protein from S. Typhimurium in E. coli.

Depositor

Joel Kralj

Article

Bruni et al Elife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

mgtC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Bba_J23118 (sigma 70)

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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