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Plasmid#115660PurposeExpresses AcrVA1 in bacteria via T7 RNAP, for purificationDepositorInsertAcrVA1
UseTagsMBP-TEVExpressionBacterialMutationPromoterT7 RNAPAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
L0-I18 Ub-Arg GG1-GG2
Plasmid#195899PurposeLevel 0 Ubiquitin-Arg tag, protein degration tag with GG1 GG2 golden gate linkersDepositorInsertUb-Arg tag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSHS212
Plasmid#87346PurposeCysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmidDepositorInsertCas9
UseTagsHis-MBPExpressionBacterialMutationC80S/C574SPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVC_CRISPR_Cat
Plasmid#120368PurposeReporter plasmid for full-site selection assayDepositorInsertCRISPR_Cat
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW204
Plasmid#113244PurposeE. coli expression vector for IHFa F13A + IHFbDepositorInsertsUseTagsExpressionBacterialMutationF13APromoterAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBA788
Plasmid#186243Purposesoc-S HR Donor Vector (T4 phage editing)DepositorInsertBBR1 backbone + T4 phage homology
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAW206
Plasmid#113246PurposeE. coli expression vector for IHFa K15A + IHFbDepositorInsertsUseTagsExpressionBacterialMutationK15APromoterT7Available sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCF823_EFS-Cas9-PuroR_lenti
Plasmid#138317PurposeLenti expression of SpyCas9 with Puro selectionDepositorInsertSpyCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAW212
Plasmid#113234PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->CCATCDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->CCATCPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
His_CL7_2NLS_iCas12a-4NLS
Plasmid#222971PurposeBacterial expression of NLS-rich iCas12a constructDepositorInsertHis_CL7_2NLS_iCas12a-4NLS
UseCRISPRTagsCL7 and HisExpressionBacterialMutationPromotertacAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1P63
Plasmid#195938PurposePhiC31-intein(DnaE-Npu) and Intein(DnaE-Ssp)-PhiC31 : pUBQ10-PI-tUBQ1-p35S-IP-t35SDepositorInsertpUBQ10-PI-tUBQ1-p35S-IP-t35S
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-I2 pPP2AA3-mutated GG0-GG1
Plasmid#195891PurposeLevel 0 pPP2AA3 promoter with GG0 and GG1 golden gate linkersDepositorInsertpPP2AA3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-T1 Target PhiC31
Plasmid#195890PurposeLevel 0 PhiC31 target: attP_PhiC31-pUBQ10-attB_PhiC31 with golden gate linker in pBLUNT plasmid.DepositorInsertPhiC31 Target
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I38 BpiI-pUBQ10-BpiI
Plasmid#195888PurposeLevel 0 pUBQ10 promoter between BpiI sites for target constructionDepositorInsertpUBQ10
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
L1P5
Plasmid#195920PurposeLevel 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-NLS-DST-tUBQ1DepositorInsertpPP2AA3-PhiC31-NLS-DST-tUBQ1
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
L1P19
Plasmid#195924PurposeLevel 1 PhiC31 cassette with constitutive promoter: pUBQ10-PhiC31-NLS-DST-tUBQ1DepositorInsertpUBQ10-PhiC31-NLS-DST-tUBQ1
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
L1P24
Plasmid#195927PurposeLevel 1 PhiC31 cassette with constitutive promoter: p35S-PhiC31-NLS-DST-tUBQ1DepositorInsertp35S-PhiC31-NLS-DST-tUBQ1
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2121
Plasmid#190343PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (empty vector control)DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I9 PhiC31 GG1-GG4
Plasmid#195892PurposeLevel 0 PhiC31 integrase with GG1 and GG4 golden gate linkersDepositorInsertPhiC31 integrase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBA791
Plasmid#186246Purposednap-S HR Donor Vector (T4 phage editing)DepositorInsertBBR1 backbone + T4 phage homology
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only