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Showing: 961 - 980 of 13178 results

  4. pCW57-GFP-2A-Cas9

    Plasmid
    #170805
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of Cas9 in combination with turbo GFP using the P2A self-cleaving peptide
    Depositor
    Morten Frodin
    Article
    Niu et al Nat Genet. 2022 Dec 5. doi: 10.1038/s415
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170805-plasmid-map-sequence-id-338370

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  8. 3xFLAG-dCas9/pMSCVhyg

    Plasmid
    #134323
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134323-plasmid-map-sequence-id-430622

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  9. pCENPTΔC-dCas9-3xGFP

    Plasmid
    #198325
    Purpose
    Expresses CENPTΔC-dCas9-3xGFP protein in mammalian cells. When coupled with a guide RNA against a high repeat target locus, this can be applied to seed ectopic kinetochores at the target locus
    Depositor
    Sarah McClelland
    Article
    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:
    Insert
    CENPTΔC (CENPT Human)
    Use
    Lentiviral
    Tags
    EGFP x 3 and dCas9
    Expression
    Mammalian
    Mutation
    truncation - encodes only amino acids 1-375
    Promoter
    CMV-TetO
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198325-plasmid-map-sequence-id-390299

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  11. p-dCas9-LSD1-Hygro

    Plasmid
    #104406
    Purpose
    transient expression of dCas9-LSD1 fusion protein
    Depositor
    Stephan Beck
    Insert
    LSD1 (KDM1A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    IRATp970A0364D (Source Bioscience) has a P405H ch…
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104406-plasmid-map-sequence-id-204465

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Showing: 961 - 980 of 13178 results