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Plasmid#119057PurposePromoter trpC from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.DepositorInsertPromoter trpC
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
FB002
Plasmid#119676PurposeTerminator tub from Neurospora crassa domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformationDepositorInsertTerminator tub
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
FB008
Plasmid#119706PurposeTerminator trpC from Aspergillus nidulans domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformationDepositorInsertTerminator trpC
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_altPKA-Cα-mPAGFP
Plasmid#114133PurposeAlternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertTestis only splice variant of mouse PKA catalytic subunit α that has no myristoylation site, C-terminally tagged by mPAGFP (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_nmPKA-Cα-mPAGFP
Plasmid#114131PurposeMammalian expression of the G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertof the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with G2A mutation (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-memRFP-3xPax7gRNA
Plasmid#224569PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.DepositorInsertmRFP1
UseCRISPRTagsMembrane localization signalExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)
Plasmid#224512PurposeA Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)DepositorInsert3xHA-BirA-T2A-mCherry-Caax
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dyn1 R271E D291R
Plasmid#198342PurposeMammalian expression plasmid of GFP-chimera proteins.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationR271E D291RPromoterCMVAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dyn1 R271E
Plasmid#198343PurposeMammalian expression plasmid of GFP-chimera proteins.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationR271EPromoterCMVAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dyn1 R271E E720R
Plasmid#198344PurposeMammalian expression plasmid of GFP-chimera proteins.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationR271E D291RPromoterCMVAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
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Dyn1 D406A
Plasmid#198341PurposeMammalian expression plasmid of GFP-chimera proteins.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationD406APromoterCMVAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8(L55W T56A V61W R65A)-MET15
Plasmid#207054PurposeExpression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
UseTags3xVSVExpressionYeastMutationL55W T56A V61W R65APromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pS238D1::sptse5-CT
Plasmid#192959PurposeFor expression of Tse5-CT (1169-1317) in P. putida with the PelB signal peptide fused at the N-terminus in biological function studiesDepositorInsertsptse5-CT
UseTagsPelB signal peptideExpressionBacterialMutationencodes for tse5 (1169-1317)PromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-RG-Linker-eGFP
Plasmid#176071PurposeEGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassetteDepositorInsertRG
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-POLB-KO-g1
Plasmid#176090PurposeCas9 plus POLB gRNA #1; contains a puromycin resistance cassetteDepositorInsertPOLB sgRNA #1 (POLB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-FHA-Linker-eGFP
Plasmid#176065PurposeEGFP fused to the C-terminus of a FHA domain & a hygromycin resistance cassetteDepositorInsertFHA
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-Miro1 (412-618)
Plasmid#127628PurposeGTPase2 domain of human Miro1 including transmembrane domain and C-terminal amino acidsDepositorInsertMiro1 (RHOT1 Human)
UseTagseGFPExpressionMammalianMutation412-618 onlyPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(S11D)-mPAGFP
Plasmid#114135PurposeMammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cyt-AKAR5
Plasmid#114138PurposeMammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5 with C-terminal nuclear export signal (NES)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only