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pRSET FLIPE intramol-10u

Plasmid

#13550

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIP-E intramol A207R (gltI E. coli)

Use

Tags

EYFP

Expression

Bacterial

Mutation

A207R, ECFP insert in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pET-CV-VAMP1

Plasmid

#168022

Purpose

Expresses eCFP-VAMP1(T29-K89)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP1(T29-K89)-mVenus

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET-CV-VAMP4

Plasmid

#168025

Purpose

Expresses eCFP-VAMP4(P48-R107)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP4(P48-R107)-mVenus

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET-CV-VAMP3

Plasmid

#168024

Purpose

Expresses eCFP-VAMP3(S11-R69)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP3(S11-R69)-mVenus

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET-CV-VAMP7

Plasmid

#168027

Purpose

Expresses eCFP-VAMP7(K121-R180)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP7(K121-R180)-mVenus

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET-CV-VAMP5

Plasmid

#168026

Purpose

Expresses eCFP-VAMP5(M1-Q60)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP5(M1-Q60)-mVenus

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJL1 Aquamarine

Plasmid

#106285

Purpose

Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Aquamarine

Use

Tags

6xHis and TEV cleavage (N terminal on insert)

Expression

Bacterial

Mutation

T65S and H148G

Promoter

T7

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-calpainsensor

Plasmid

#36182

Purpose

detection of activity of ubiquitous calpains

Depositor

Isabelle Richard

Article

Stockholm et al J Mol Biol. 2005 Feb 11;346(1):215-22. E

Insert

alpha-Fodrin cleavage site (Sptan1 Mouse)

Use

Tags

eCFP and eYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-InPAkt

Plasmid

#181914

Purpose

Indicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.

Depositor

Jin Zhang

Article

Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

Insert

InPAkt

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET-CVm-PTEN

Plasmid

#168032

Purpose

Expresses eCFP-PTEN(M265-N329)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-PTEN(M265-N329)-mVenus

Use

Tags

Hisx6 and myc

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-PlcR(K30/32L)

Plasmid

#181831

Purpose

Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR (K30L,K32L)

Use

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-JNKAR1-NES

Plasmid

#84632

Purpose

Unimolecular, FRET-based JNK activity reporter; cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

ECFP-FHA1-JNKsubDD-Citrine-NES

Use

Tags

6xHis, Citrine, ECFP, Nuclear export signal (NES)…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pRSM12

Plasmid

#101643

Purpose

Encodes the fluorescent protein meffCFP for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

meffCFP

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta11 Aphrodite

Plasmid

#13569

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta11 Aphrodite

Use

Tags

Aphrodite and ECFP

Expression

Bacterial

Mutation

Fluorophore-derived linker shortened. F16A mutati…

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pRSM14

Plasmid

#101645

Purpose

Encodes the fluorescent protein efasCFP for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

efasCFP

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta13 Aphrodite

Plasmid

#13570

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta13 Aphrodite

Use

Tags

Aphrodite and ECFP

Expression

Bacterial

Mutation

Fluorophore-derived linker shortened. F16A mutati…

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pET-CVm-Sec22b

Plasmid

#168029

Purpose

Expresses eCFP-Sec22b(A131-H190)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-Sec22b(A131-H190)-mVenus

Use

Tags

Hisx6 and myc

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET-CVm-VAMP8

Plasmid

#168028

Purpose

Expresses eCFP-VAMP8(G7-R67)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP8(G7-R67)-mVenus

Use

Tags

Hisx6 and myc

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSM13

Plasmid

#101644

Purpose

Encodes the fluorescent protein meleCFP for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

meleCFP

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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QUAS-jGCaMP7s-T2A-tdTomato

Plasmid

#200010

Purpose

QUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2A

Depositor

Carolyn McBride

Article

Zhao et al Nature. 2022 May;605(7911):706-712. doi:

Insert

3xP3-ECFP-jGCaMP7s-td-Tomato

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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