1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. GAL-Aur1-2GFP-Hygromycin

   Plasmid

   #184775

   Purpose

   Yeast expression of Aur1 under GAL promoter. Uses antibiotic resistance marker hphMX6.

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   proGAL-Aur1 (AUR1 Budding Yeast)
   

   Use

   Tags

   2EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. puc19-pCas13a

   Plasmid

   #118963

   Purpose

   Intermediate/cloning vector that can be used for sub-cloning into final destination vectors based on one's requirements.

   Depositor

   Magdy Mahfouz

   Article

   Aman et al Genome Biol. 2018 Jan 4;19(1):1. doi: 10

   Insert

   Cas13a
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFREE_Amp

   Plasmid

   #92051

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   pRhamBAD and ptet

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAVS1-TLR6

   Plasmid

   #118997

   Purpose

   Targeting vector for human AAVS1 with TLR6 reporter insert

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   AAVS1 (PPP1R12C, AAVS1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTC217

   Plasmid

   #70018

   Purpose

   Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

   Depositor

   Daniel Voytas

   Article

   Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

   Insert

   Nuclease (Cas9/sgRNA) + Donor + GVR
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pI3-MTU-DO-G418

   Plasmid

   #160023

   Purpose

   Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). G418 selection.

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BB3rN_14*

   Plasmid

   #98550

   Purpose

   empty BB3 for insertion and overexpression of 1 gene in P. pastoris

   Depositor

   Brigitte Gasser, Diethard Mattanovich, Michael Sauer

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p426-SNR52p-gRNA.csr-1.Y-SUP4t

   Plasmid

   #68060

   Purpose

   gRNA for csr-1 locus in N. crassa

   Depositor

   Christian Hong

   Article

   Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

   Insert

   gRNA for csr-1 locus
   

   Use

   CRISPR; N. crassa, fungi

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. StrepII-tag

   Plasmid

   #55181

   Purpose

   Streptactin-tag

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   StrepII tag
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEM705 Gso (Ser)

    Plasmid

    #109353

    Purpose

    GsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gso chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cysteine at position 391 to Serine (Pertu…

    Promoter

    CAG

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSF464

    Plasmid

    #167672

    Purpose

    Intermediate vector for four gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEM705 Gsi (Ser)

    Plasmid

    #109352

    Purpose

    GsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gsi chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cys at position 391 to Serine (Pertussis …

    Promoter

    CAG

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pI2-MTU-DO-G418

    Plasmid

    #160018

    Purpose

    Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). G418 selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFBD-SpyTag-noro-VLP

    Plasmid

    #165989

    Purpose

    Expresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteins

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

    Insert

    SpyTag-noro-VLP (ORF2 norovirus, Synthetic)
    

    Use

    Tags

    SpyTag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pI4-MTU-DO-G418

    Plasmid

    #160215

    Purpose

    Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). G418 selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pZmUbi-5'UTR

    Plasmid

    #154057

    Purpose

    Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    Promoter and 5' UTR Ubiquitin (Zea mays)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFREE_Cm

    Plasmid

    #92052

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA-tag

    Plasmid

    #55182

    Purpose

    HA-tag

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    HA tag
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-Cas9-162A

    Plasmid

    #84918

    Purpose

    for in vitro transcription of Cas9 with polyA tail encoded

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Cas9
    

    Use

    Tags

    Flag, NLS, and polyA tail

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAC1430

    Plasmid

    #127981

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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