1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pTC217

   Plasmid

   #70018

   Purpose

   Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

   Depositor

   Daniel Voytas

   Article

   Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

   Insert

   Nuclease (Cas9/sgRNA) + Donor + GVR
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pR26 GFP Dest

   Plasmid

   #74283

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFBD-SpyTag-noro-VLP

   Plasmid

   #165989

   Purpose

   Expresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteins

   Depositor

   Vesa Hytönen

   Article

   Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

   Insert

   SpyTag-noro-VLP (ORF2 norovirus, Synthetic)
   

   Use

   Tags

   SpyTag

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-BCL9

   Plasmid

   #113759

   Purpose

   For the expression of FLAG-BCL9 in mammalian cells

   Depositor

   Mariann Bienz

   Article

   de la Roche et al BMC Cancer. 2008 Jul 15;8:199. doi: 10.1

   Insert

   BCL9 (BCL9 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSF464

   Plasmid

   #167672

   Purpose

   Intermediate vector for four gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pZmUbi-5'UTR

   Plasmid

   #154057

   Purpose

   Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Insert

   Promoter and 5' UTR Ubiquitin (Zea mays)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   N/A

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113343

   Purpose

   Expresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   p300core-dCas9-VP64-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFREE_Cm

   Plasmid

   #92052

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   pRhamBAD and ptet

   Available since

   Aug. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HA-tag

   Plasmid

   #55182

   Purpose

   HA-tag

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   HA tag
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SPI1_pet28a

    Plasmid

    #131653

    Purpose

    Bacterial expression of ETS gene SPI1

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAC1430

    Plasmid

    #127981

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGS-21a-PcAad1

    Plasmid

    #163039

    Purpose

    expression of aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium

    Depositor

    Jean-Marie Francois

    Article

    Yang et al BMC Microbiol. 2012 Jun 28;12:126. doi:

    Insert

    aryl-alcohol dehydrogenase
    

    Use

    Tags

    GST and His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZmUBQ:LUC

    Plasmid

    #131860

    Purpose

    Transient expression of firefly luciferase from the Zea mays ubiquitin promotor

    Depositor

    Paul Schulze-Lefert

    Article

    Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

    Insert

    luciferase
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    ZmUBQ

    Available since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZmUBQ:GW

    Plasmid

    #132358

    Purpose

    Expression of CDS from the Zea mays ubiquitin promotor

    Depositor

    Paul Schulze-Lefert

    Article

    Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

    Type

    Empty backbone

    Use

    Tags

    YFP

    Expression

    Plant

    Mutation

    Promoter

    pZmUBQ

    Available since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-Cas9-162A

    Plasmid

    #84918

    Purpose

    for in vitro transcription of Cas9 with polyA tail encoded

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Cas9
    

    Use

    Tags

    Flag, NLS, and polyA tail

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSF278

    Plasmid

    #167673

    Purpose

    Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOCC154

    Plasmid

    #118902

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    

    Use

    Tags

    TwinStrep, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHRE

    Plasmid

    #134909

    Purpose

    Overexpression of recombinant proteins in plants thanks to efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Test UTR combinations by swapping 5' and 3' UTRs.

    Depositor

    George Lomonossoff

    Article

    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    double 35S

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOCC153

    Plasmid

    #118901

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    TwinStrep, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Htb2-GFP

    Plasmid

    #117669

    Purpose

    Colorize the nuclei

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    Htb2-GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Contains the gene encoding the Histone Htb2 from …

    Promoter

    pGAP

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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