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Human a3 nicotinic receptor subunit EM construct

Plasmid

#163960

Purpose

pEZT-BM vector with alpha3 gene after CMV promoter, published EM construct

Depositor

Ryan Hibbs

Article

Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do

Insert

Human alpha3 nicotinic acetylcholine receptor subunit EM construct

Use

Baculovirus (bacmam) production and mammalian exp…

Tags

apocytochrome b(562)RIL (BRIL)

Expression

Mammalian

Mutation

Asn348-Ser402 replaced with apocytochrome b(562)R…

Promoter

CMV

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-3XHA

Plasmid

#127881

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HA tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-3XHA (PPP2CA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-FAID

Plasmid

#127885

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter FAID tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-H3F-AID (PPP2CA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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FUT8-Dox

Plasmid

#124631

Purpose

Doxycycline-inducible FUT8 expression in mammalian cells

Depositor

Ron Weiss

Article

Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

Inserts

Alpha-(1,6)-fucosyltransferase
Tetracycline repressor A3 mutant
TagFP635

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE-tight and hEF1a

Available since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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mLama1 AAV sgRNA 1, 2, 5

Plasmid

#135339

Purpose

Expression plasmid of VP64-SadCas9 sgRNA 1, 2 and 3

Depositor

Ronald Cohn

Article

Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

Insert

Lama1 VP64-dCas9 sgRNA Guides

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-hSNCA

Plasmid

#170445

Purpose

Lentiviral vector expressing human SNCA, under control of EF1alpha promoter.

Depositor

Sang-Hun Lee

Article

Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

Insert

human SNCA (SNCA Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1α-mouse-retroCHMP3-HA

Plasmid

#154178

Purpose

expresses mouse retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

retroCHMP3

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Human a4 nicotinic subunit crystallization construct

Plasmid

#85841

Purpose

pEZT-BM vector with alpha4 gene after CMV promoter, published crystallization construct

Depositor

Ryan Hibbs

Article

Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do

Insert

Human a4 nAChR subunit crystallization construct (CHRNA4 Human)

Use

Baculovirus (bacmam) production and mammalian exp…

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
iSH-iRFP670-sspB

Plasmid

#176102

Purpose

Heterodimerization with iLID, phosphatidylinositol 3-kinase derived iSH

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

phosphatidylinositol 3-kinase (Pik3r1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pChuk

Plasmid

#87033

Purpose

express IKKalpha in mammalian cells

Depositor

Georgios Stathopoulos

Article

Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

Insert

IKKalpha (Chuk Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEF1α-spider-monkey-retroCHMP3-HA

Plasmid

#154179

Purpose

expresses spider monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

retroCHMP3

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
V5V

Plasmid

#29423

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus-5-Venus

Use

Anisotropy and brightness standard

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
VVV

Plasmid

#27814

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus-5-Venus-6-Venus

Use

Anisotropy and brightness standard

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
V17V

Plasmid

#29424

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus-17-Venus

Use

Anisotropy and brightness standard

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
V32V

Plasmid

#29561

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus-32-Venus

Use

Anisotropy and brightness standard

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
pLMB509

Plasmid

#40084

Depositor

Philip Poole

Article

Tett et al Appl Environ Microbiol. 2012 Oct;78(19):

Inserts

gfpmut 3.1
tauR
tau promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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VVVVVV

Plasmid

#27813

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus-5-Venus-5-Venus-5-Venus-5-Venus-5-Venus

Use

Anisotropy and brightness standard

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
VVVVV

Plasmid

#29426

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus-5-Venus-5-Venus-5-Venus-5-Venus

Use

Anisotropy and brightness standard

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
VVVV

Plasmid

#29425

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus-5-Venus-5-Venus-6-Venus

Use

Anisotropy and brightness standard

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
CMV-mCh-kCAAX

Plasmid

#112267

Purpose

ITRACK Acceptor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

mCherry with KRas membrane targeting domain

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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