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pcDNA5FRT/TO-p97-RH-mycStrep

Plasmid

#31839

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Use

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

R155H

Promoter

CMV

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pOmpT

Plasmid

#112123

Purpose

Bacterial expression outer membrane protein T (OmpT), wild type

Depositor

Karen Fleming

Article

Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

Insert

OmpT (ompT E. coli)

Use

Tags

Expression

Bacterial

Mutation

N-terminal signal sequence not included and repla…

Promoter

T7

Available since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Cx32-7

Plasmid

#55287

Purpose

Localization: Gap Junctions, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Cx32 (GJB1 Human)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pKF-D2irTNP2AG-T2APuroR-5kwh

Plasmid

#128255

Purpose

Mammalian linearizer gene circuit based on negative feedback in a Flp-In expression system controlling the PuroR gene.

Depositor

Gabor Balazsi

Article

Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

Insert

hTetR::P2A::EGFP::T2A::PuroR

Use

Synthetic Biology; Flp-in expression vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV-D2ir promoter

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRJPaph-HcRed

Plasmid

#67035

Purpose

Plasmid for Paph-HcRed integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
HcRed

Use

Conjugative bacterial vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMIR-D10 mutUTR

Plasmid

#19116

Depositor

Bob Weinberg

Article

Ma et al Nature. 2007 Oct 11. 449(7163):682-8.

Insert

HOXD10 mut 3' UTR (HOXD10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
pCEP4-Q769gp160

Plasmid

#100928

Purpose

Mammalian expression plasmid for Env from the Q769.d22 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q769.d22) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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UKNP6.1.2

Plasmid

#98388

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2d10-364delta263-295-TEV-STREP

Plasmid

#171830

Purpose

Mammalian expression of human WIPI2d 10-364 delta263-295 with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep

Expression

Mammalian

Mutation

10-364delta263-295

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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MEIS1A-pET

Plasmid

#21015

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1A (Meis1 Mouse)

Use

Tags

HIS and T7

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pBlueScript II SK-SNAP donor for H3f3b

Plasmid

#186933

Purpose

Genomic targeting of SNAP tag at H3f3b C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

SNAP donor for H3f3b (H3f3b Mouse)

Use

Tags

SNAP tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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UKNP2.2.1

Plasmid

#98209

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pK RagA-RagC

Plasmid

#99649

Purpose

Bacterial expression of wildtype Rag GTPase dimer

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Use

Tags

10x His tag, Poly arginine linker, and SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFE-vcDAB

Plasmid

#133004

Purpose

pFE carrying the vcdabA2 ( locus_tag: CSW01_RS07960) and vcdabB2 genes (locus_tag: CSW01_RS07955)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

vcdabB
vcdabA

Use

Tags

Expression

Bacterial

Mutation

Promoter

tetR

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pShuttle-FEN1deltaP

Plasmid

#35030

Depositor

Sheila Stewart

Article

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1DELTAP (FEN1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

8 AMINO ACID DELETION OF P BOX (amino acids 337–3…

Promoter

CMV

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pVRa34_1384

Plasmid

#49693

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF34_1384

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pOmpW

Plasmid

#112121

Purpose

Bacterial expression of outer membrane protein W (OmpW), wild type

Depositor

Karen Fleming

Article

Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

Insert

OmpW (ompW E. coli)

Use

Tags

Expression

Bacterial

Mutation

N-terminal signal sequence not included and repla…

Promoter

T7

Available since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 AT1AR-324delta

Plasmid

#45635

Depositor

Robert Lefkowitz

Article

Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

Insert

AT1AR-324delta (Agtr1a Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

added stop codon at 324 aa

Promoter

CMV

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pWaldo-GFPe_PepTSo

Plasmid

#58334

Purpose

Expresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged protein

Depositor

Simon Newstead, So Iwata

Article

Newstead et al EMBO J. 2011 Jan 19;30(2):417-26. doi: 1

Insert

PepTso

Use

Tags

GFP-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
UKNP2.5.1

Plasmid

#98213

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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