Addgene

We narrowed to 14,839 results for: grna

Showing: 9761 - 9780 of 14839 results

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pU6-EF-gAAVS1

Plasmid

#112262

Purpose

Express g-AAVS1

Depositor

Zhili Rong

Article

Ma et al FEBS Lett. 2018 Jun 5. doi: 10.1002/1873

Insert

g-AAVS1

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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PM-NM!TB

Plasmid

#48652

Purpose

Bacterial NM crRNA expression: targets NM to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial NM crRNA to prototspacer B

Use

CRISPR

Tags

Expression

Mutation

Promoter

J23100 promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-PM mir shHoxB9 b

Plasmid

#27020

Depositor

Joan Massague

Article

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

mir HoxB9 3b

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pMM732

Plasmid

#68897

Purpose

IPTG-inducible CRISPRi vector targeting NanoLuc (NL2), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Christopher Voigt, Timothy Lu

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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PM-SP!TA

Plasmid

#48649

Purpose

Bacterial SP crRNA expression: targets SP to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer A

Use

CRISPR

Tags

Expression

Mutation

Promoter

J23100 promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shISCU

Plasmid

#102972

Purpose

Suppression of ISCU

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shISCU

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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PSUPER-1660s(hPot1)

Plasmid

#11132

Depositor

Christopher Counter

Article

Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

Insert

RNAi vector for human protection of telomeres 1(control for 1660) (POT1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pLKO.1-blast-cfRab25_4

Plasmid

#26712

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

Insert

dog Rab25 RNAi

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Canine Rab25 RNAi.

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
PM-ST1!TA

Plasmid

#48653

Purpose

Bacterial ST1 crRNA expression: targets ST1 to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial ST1 crRNA to prototspacer A

Use

CRISPR

Tags

Expression

Mutation

Promoter

J23100 promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-cfRabin8_5

Plasmid

#26714

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

Insert

dog Rabin8 RNAi

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Dog Rabin8 alpha isoform RNAi

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-cfRabin8_4

Plasmid

#26713

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

Insert

dog Rabin8 RNAi

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

RNAi to dog Rabin8 alpha isoform.

Promoter

Available since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-blast-cfRab25_2

Plasmid

#26711

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

Insert

dog Rab25 RNAi

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

RNAi to canine Rab25.

Promoter

Available since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only

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pRC065d

Plasmid

#225979

Purpose

dRxCas13d expressed from J23107 promoter with J23119-driven crRNA (non-cognate DR)

Depositor

Jesse Zalatan

Article

Cardiff et al Nucleic Acids Res. 2024 May 22;52(9):540

Inserts

dRxCas13d
buj1 crRNA (non-cognate DR) targets the unstructured RBS

Use

CRISPR

Tags

Expression

Mutation

Promoter

J23107 and J23119

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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p8390 LentiCRISPRv2 Neo sgNT-2

Plasmid

#221650

Purpose

Expression of non-targeting control sgRNA

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

non-targeting sgNT-2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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OgeuIscB ωRNA-v2

Plasmid

#220958

Purpose

human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloing

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

Insert

OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-Vamp2-GeneTRAP

Plasmid

#218187

Purpose

To KO and genetrap mouse Vamp2 simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Vamp2 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-Vamp2-Tag-HA

Plasmid

#218188

Purpose

To tag endogenous mouse VAMP2 with HA

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Vamp2 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAGK43

Plasmid

#222223

Purpose

Edits NPAS2 Gene.

Depositor

Seth Shipman

Article

Crawford et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

Eco1 RT; 112 nt donor; PAM -19 (NPAS2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ141B-ge4.1

Plasmid

#205144

Purpose

Guide RNA expression cassette harboring AtU3 promoter, ge4.1 5’ modification, and ge4.1 3’ modification

Depositor

Yiping Qi

Article

Tang et al Plant Genome. 2024 May 28:e20465. doi: 1

Insert

gRNA cloning site

Use

CRISPR

Tags

Expression

Mutation

Promoter

AtU3

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL391

Plasmid

#219501

Purpose

Express Ade2 and Faa1 editrons from a single Gal7 promoter, separated by a Csy4 recognition sequence

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

Insert

Eco1 editing ncRNAs and Cas9 gRNAs to edit ADE2 and FAA1

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL7

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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