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pET19b-rShHTL7 cPGFP(C164)

Plasmid

#166002

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rShHTL7 cPGFP(C164)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-DAD2 cPGFP(N180)

Plasmid

#166000

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

DAD2 cPGFP(N180)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-DAD2 cPGFP(A165)

Plasmid

#165999

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

DAD2 cPGFP(A165)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-HisDAD2 cPGFP(G164)

Plasmid

#165997

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

HisDAD2 cPGFP(G164)

Use

Tags

6His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-rShHTL7 cPGFP(L166)

Plasmid

#165995

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rShHTL7 cPGFP(L166)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-DAD2 cPGFP(G164)

Plasmid

#165993

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

DAD2 cPGFP(G164)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 SEC66-VAC8-GFP

Plasmid

#166840

Purpose

Expresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8, SEC66 Budding Yeast)

Use

Tags

GFP and SEC66 transmembrane domain

Expression

Yeast

Mutation

SEC66 transmembrane domain

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 VAC8-G2A-GFP

Plasmid

#166837

Purpose

Expresses a myristoylation mutant Vac8-GFP in yeasts, mutation is Gly2Ala

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

G2A

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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mCerulean3-31aa-Venus-pEGFP-C3

Plasmid

#166754

Purpose

For expression of mCerulean3 linked to Venus by 31 amino acid linker

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

mCerulean3-31aa-Venus

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-BimEL(Bik)-pEGFP-C1

Plasmid

#166760

Purpose

To examine effect of swapping the BH3 region in full length BimEL with that of Bik

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

BimEL(Mus musculus)-(Bik BH3) (Bcl2l11, BIK Mouse, Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

BH3 region removed and inserted BH3 of human Bik …

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-BimEL(Bid)-pEGFP-C1

Plasmid

#166759

Purpose

To examine effect of swapping the BH3 region in full length BimEL with that of Bid

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

BimEL(Bid) (Bcl2l11, Bid Mouse)

Use

Tags

Venus

Expression

Mammalian

Mutation

BH3 region removed and inserted BH3 of mouse Bid …

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-BimEL(Bad)-pEGFP-C1

Plasmid

#166758

Purpose

To examine effect of swapping the BH3 region in full length BimEL with that of Bad

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

BimEL(Mus musculus)-(Bad BH3) (Bcl2l11, BAD Mouse, Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

BH3 region removed and inserted BH3 of human Bad …

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-f1(-)-GAPDH-IRES-EGFP

Plasmid

#115918

Purpose

donor to insert IRES-EGFP at the GAPDH locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

IRES-EGFP

Use

Donor template

Tags

dTomato

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-GFP-mirMecp2

Plasmid

#163704

Purpose

pAAV plasmid to induce expression of micro-RNA targeted against mouse Mecp2 and EGFP

Depositor

Michisuke Yuzaki

Article

Ito-Ishida et al J Neurosci. 2020 Nov 4;40(45):8746-8766.

Insert

mirMecp2 and EGFP (Mecp2 Aequorea victoria, Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-GFP-mirNega

Plasmid

#163705

Purpose

pAAV plasmid to induce expression of universal negative control micro-RNA and EGFP

Depositor

Michisuke Yuzaki

Article

Ito-Ishida et al J Neurosci. 2020 Nov 4;40(45):8746-8766.

Insert

mirNega and EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-KAR2-mEGFP-HDEL

Plasmid

#160467

Purpose

Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to mEGFP with an ER retrieval signal

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

KAR2-mEGFP-HDEL

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_vhhGFP4::T48-Baz::mCherry

Plasmid

#163927

Purpose

Expression of GrabFP-A-extracellular under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_mCherry::T48-Baz::vhhGFP4

Plasmid

#163928

Purpose

Expression of GrabFP-A-intracellular under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Mutation

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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GSX2-P2A-tGFP-DTA

Plasmid

#161750

Purpose

tGFP plasmid for the c-terminal targeting of human GSX2 locus

Depositor

Elena Cattaneo

Article

Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

Insert

turboGFP

Use

CRISPR

Tags

P2A-tGFP

Expression

Mammalian

Mutation

Promoter

endogenous GSX2 promoter

Available since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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hPGK-ABE7.10-P2A-GFP

Plasmid

#162511

Purpose

Express ABE7.10-P2A-GFP by hPGK promoter in mammalian cells

Depositor

Yongsub Kim

Article

Shin et al Nucleic Acids Res. 2021 Feb 26;49(4):239

Insert

ABE7.10-P2A-GFP

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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