-
-
hGli1 flag3x
Plasmid#84922Purposemammalian expression of Gli1DepositorInsertGli1 (GLI1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PYGL_WT_V5
Plasmid#82991PurposeGateway Donor vector containing PYGL, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertPYGL (PYGL Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
hTRAF2pLPCX-HA-Flag/P874
Plasmid#20229DepositorInsertTNF receptor-associated factor 2 (TRAF2 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationunmutated NM_021138.3. L replaces M at beginning…PromoterAvailable sinceMarch 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRpHLuorin2-N1
Plasmid#171716PurposeRatiometric pHLuorin2 backbone (C terminal fusion)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Venus-Fluc (puro)
Plasmid#140328PurposeLentivirus for expression of Venus-Fluc (firefly luciferase) fusion proteinDepositorInsertVenus-Firefly Luciferase (luc2)
UseLentiviralTagsExpressionMutationPromoterhuman PGK promoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
NLS-LucWT-GFP
Plasmid#127709PurposeThe expressed ORF encodes nucleus location signal with GFP and WT Luciferase.DepositorInsertLuciferase
UseTagsEGFP and FLAG-NLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTracer-HV1
Plasmid#24710DepositorInsertHV1 (HVCN1 Human)
UseTagsGFP--NOT a fusion!ExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1
Plasmid#81057Purposemammalian expression of Zdk1DepositorInsertZdk1
UseTags6x His tag, FLAG, and mCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
BPK1520
Plasmid#65777PurposeHuman expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNADepositorInsertSpCas9 gRNA backbone, without spacer sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only
