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pKHH030_sgAMBRA1#2

Plasmid

#174147

Purpose

Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pKHH030_sgAMBRA1#1

Plasmid

#174146

Purpose

Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pINTO-N3::hTET3 V908L

Plasmid

#136370

Purpose

Expresses epitope-tagged human TET3 V908L variant in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Use

Tags

2xStrep-tagII, FLAG, and HA

Expression

Mammalian

Mutation

V908L

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pINTO-N3::hTET3 V1089M

Plasmid

#136369

Purpose

Expresses epitope-tagged human TET3 V1089M variant in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Use

Tags

2x Strep-tagII, FLAG, and HA

Expression

Mammalian

Mutation

V1089M

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pINTO-N3::hTET3 A1076T

Plasmid

#136368

Purpose

Expresses epitope-tagged human TET3 A1076C variant in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Use

Tags

2xStrepTagII, FLAG, and HA

Expression

Mammalian

Mutation

A1076T

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pINTO-N3::hTET3 R752C

Plasmid

#136367

Purpose

Expresses epitope-tagged human TET3 R752C variant in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Use

Tags

2xStrepTagII, FLAG, and HA

Expression

Mammalian

Mutation

R752C

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pINTO-N3::hTET3 F1072C

Plasmid

#136366

Purpose

Expresses epitope-tagged human TET3 F1072C variant in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Use

Tags

2xStrepTagII, FLAG, and HA

Expression

Mammalian

Mutation

F1072C

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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MBP-rPKL S12TAG

Plasmid

#107155

Purpose

MBP-rPKL construct for production and purification of rat PKL pS12

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Use

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Mutation

Serine 12 to TAG for phosphoserine insertion

Promoter

PBAD

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-rPKL S113TAG

Plasmid

#107157

Purpose

MBP-rPKL S113TAG for production and purification of rat PKL pS113

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Use

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Mutation

Serine 113 changed to TAG for phosphoserine inser…

Promoter

PBAD

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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Fibronectin-human-mutated N-terminal (N) O-Glycosylation site

Plasmid

#120404

Purpose

enables eukaryotic expression of human plasma fibronectin in which the first O-glycosylation site was mutated from threonine to serine

Depositor

Inaam Nakchbandi

Article

Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.

Insert

Fibronectin (FN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Containing complete variable region with a mutati…

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Fibronectin-human-mutated V2 O-Glycosylation site

Plasmid

#120405

Purpose

enables eukaryotic expression of human plasma fibronectin in which the second O-glycosylation site was mutated from threonine to serine

Depositor

Inaam Nakchbandi

Article

Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.

Insert

Fibronectin (FN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Contains complete variable region with a mutation…

Promoter

CMV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSIREN-RetroQ-BPTF-sh5

Plasmid

#83045

Purpose

MMLV LTR plasmid pSIREN-RetroQ-BPTF-sh5

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA #5 to BPTF (BPTF, Bptf Human, Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.3_SARS2_omicron BA.2

Plasmid

#183700

Purpose

expressing SARS-CoV-2 BA.2 spike protein for pseudovirus production

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

Spike (BA.2) (S SARS-CoV-2)

Use

Lentiviral and Retroviral

Tags

Expression

Mammalian

Mutation

c-terminal 18 aa deletion

Promoter

CMV

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-NL63-D14

Plasmid

#172666

Purpose

encodes NL63 spike protein, for pseudovirus production

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

HCNV63gp2 (HCNV63gp2 human coronavirus NL63)

Use

Lentiviral and Retroviral

Tags

NA

Expression

Mammalian

Mutation

c-terminal deletion of 14aa

Promoter

CMV

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK2-R67S/S153D

Plasmid

#213597

Purpose

Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK1 (MAPK1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R67S, S153D

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_omicron_BA.1

Plasmid

#180375

Purpose

Pseudovirus plasmid expressing SARS-CoV-2 omicron BA.1 strain spike protein

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

Spike (omicron BA.1 variant) (S SARS-CoV-2)

Use

Lentiviral and Retroviral

Tags

Expression

Mammalian

Mutation

c terminal D18

Promoter

CMV

Available since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-TAF15 Stop

Plasmid

#84895

Purpose

DONOR vector for Gateway cloning of TAF15

Depositor

Aaron Gitler

Article

Couthouis et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

TAF15 (TAF15 Human)

Use

Gateway donor

Tags

Expression

Mutation

Promoter

none

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJOP-HTT-HR65Q

Plasmid

#92249

Purpose

HTT donor DNA with 65Q for homologous recombination-mediated gene modification

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

HTT donor DNA (HTT Human)

Use

Precisionx hr targeting vector

Tags

Expression

Mutation

Barcode of wobble bases to prevent TALEN cleaving…

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_BA.4/5

Plasmid

#186810

Purpose

pseudovirus plasmid expressing BA.4 and BA.5 spike protein

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

43740568 (S SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

HY69-70del, L452R, F486V, Q493R, on the basis of …

Promoter

CMV

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pJOP-HTT-HR45Q

Plasmid

#92248

Purpose

HTT donor DNA with 45Q for homologous recombination-mediated gene modification

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

HTT donor DNA (HTT Human)

Use

Precisionx hr targeting vector

Tags

Expression

Mutation

Barcode of wobble bases to prevent TALEN cleaving…

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge