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Plasmid#186742PurposeDox inducible CSK-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorInsertCSK (CSK Human)
UseLentiviralTagsP2A-mCherryExpressionMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-δ
Plasmid#197259PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-3 δ (AP3D1 Human)
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationVal 549 Ile substitution; silent substitutions in…PromoterADH1Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ3A
Plasmid#197513PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-3 σ3A
UseTagsGAL4-DNA binding domain fragment, HA tag, and nuc…ExpressionYeastMutationsilent substitution in codon 2 of tyrosinase tail…PromoterADH1 and MET25Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1149-1376_AC
Plasmid#148475PurposeInsect Expression of DmNot1_1149-1376DepositorInsertDmNot1_1149-1376 (Not1 Fly)
UseTagsExpressionInsectMutation1 silent mutation compared to the sequence given …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmMe31B-Q294AN296F-dsRNAres_W
Plasmid#147862PurposeInsect Expression of DmMe31B-Q294AAN296F-dsRNAresDepositorInsertDmMe31B-Q294AAN296F-dsRNAres (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmMe31B-Q294AN296FR347A-dsRNAres_W
Plasmid#147863PurposeInsect Expression of DmMe31B-Q294AAN296FR347A-dsRNAresDepositorInsertDmMe31B-Q294AAN296FR347A-dsRNAres (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmMe31B-R347A-dsRNAres_W
Plasmid#147864PurposeInsect Expression of DmMe31B-R347A-dsRNAresDepositorInsertDmMe31B-R347A-dsRNAres (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmMe31B-S291A-dsRNAres_W
Plasmid#147865PurposeInsect Expression of DmMe31B-S291A-dsRNAresDepositorInsertDmMe31B-S291A-dsRNAres (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-del1967-2251-dsRNAres_U
Plasmid#147711PurposeInsect Expression of DmNot1-del1967-2251-dsRNAresDepositorInsertDmNot1-del1967-2251-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-del1147-1717-dsRNAres_R
Plasmid#147434PurposeInsect Expression of DmNot1-del1147-1717-dsRNAresDepositorInsertDmNot1-del1147-1717-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation8 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1-1709-dsRNAres_R
Plasmid#147430PurposeInsect Expression of DmNot1_1-1709-dsRNAresDepositorInsertDmNot1_1-1709-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation6 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-del942-971-GGSG_M
Plasmid#146950PurposeInsect Expression of DmAGO1-del942-971-GGSGDepositorInsertDmAGO1-del942-971-GGSG (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-del942-971-GSG_M
Plasmid#146951PurposeInsect Expression of DmAGO1-del942-971-GSGDepositorInsertDmAGO1-del942-971-GSG (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-del835-886-GGSG_M
Plasmid#146948PurposeInsect Expression of DmAGO1-del835-886-GGSGDepositorInsertDmAGO1-del835-886-GGSG (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-del835-886-GSG_M
Plasmid#146949PurposeInsect Expression of DmAGO1-del835-886-GSGDepositorInsertDmAGO1-del835-886-GSG (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsNot4_1-274-GB1His6_AG
Plasmid#148783PurposeBacterial Expression of HsNot4_1-274DepositorInsertHsNot4_1-274 (CNOT4 Human)
UseTagsExpressionBacterialMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsGIGYF2_35-54-GB1_AE
Plasmid#148558PurposeBacterial Expression of HsGIGYF2_35-54DepositorInsertHsGIGYF2_35-54 (GIGYF2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsGIGYF2_35-72-GB1_AE
Plasmid#148559PurposeBacterial Expression of HsGIGYF2_35-72DepositorInsertHsGIGYF2_35-72 (GIGYF2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsGIGYF2_35-105-GB1_AE
Plasmid#148560PurposeBacterial Expression of HsGIGYF2_35-105DepositorInsertHsGIGYF2_35-105 (GIGYF2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-CvH-Hs4EHP_1-234_AE
Plasmid#148561PurposeBacterial Expression of Hs4EHP_1-234DepositorInsertHs4EHP_1-234 (EIF4E2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only