pGADT7-δ
(Plasmid #197259)

• Purpose: Expression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
• Depositing Lab: Juan Bonifacino
• Publication: Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30. doi: 10.1074/jbc.M110.197178. Epub 2010 Nov 19.

Backbone

• Vector backbone: pGADT7
• Backbone size w/o insert (bp): 7987
• Total vector size (bp): 11451
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AP-3 δ
• Species: H. sapiens (human)
• Insert Size (bp): 3462
• Mutation: Val 549 Ile substitution; silent substitutions in codons 663, 867 and 876
• Entrez Gene: AP3D1 (a.k.a. ADTD, HPS10, hBLVR)
• Promoter: ADH1
• Tags / Fusion Proteins:
  • GAL4 transcriptional activation domain (AD) fragment (N terminal on backbone)
  • HA tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: GAL4 transcriptional activation domain forward
• 3′ sequencing primer: GAL4 transcriptional activation domain reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry