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Showing: 9661 - 9680 of 51990 results

  1. HRE:GFP-pGK:Cre

    Plasmid
    #68552
    Purpose
    Lentivirus expressing hypoxia-responsive GFP reporter and Cre
    Depositor
    Tyler Jacks
    Inserts
    Green Fluorescent Protein
    Cre
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    HRE and pGK
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    68552-plasmid-map-sequence-id-120714

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  2. pUbi_dCas9-BFP-KRAB-gRNA

    Plasmid
    #221503
    Purpose
    CRISPRi machinery fish codon optimized expressing plasmid
    Depositor
    Alessandro Ori
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Tags
    Expression
    Mutation
    D10A and H839A point mutations
    Promoter
    Zebrafish Ubiquitin promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221503-plasmid-map-sequence-id-439415

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  3. pTRE3G-VP64-dCas9-VP64-ZsG

    Plasmid
    #192668
    Purpose
    Dox-inducible expression of VP64-dCas9-VP64 with 2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    VP64-dCas9-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    TRE3G
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192668-plasmid-map-sequence-id-379892

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  4. pPB-TetON-mEGFP-MYOD1_AroPERFECT

    Plasmid
    #215620
    Purpose
    For integration of MYOD1 AroPERFECT T2A mEGFP
    Depositor
    Denes Hnisz
    Insert
    MYOD1 AroPERFECT (MYOD1 Human)
    Use
    Tags
    T2A mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    215620-plasmid-map-sequence-id-426936

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  5. pENTR ARRET

    Plasmid
    #111384
    Purpose
    ARRET with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences
    Depositor
    Klaus Holzmann
    Insert
    ARRET
    Use
    Gateway entry cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    111384-plasmid-map-sequence-id-209223

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  6. pPB-CAG-Venus-Akaluc

    Plasmid
    #209768
    Purpose
    Venus-Akaluc is inserted into pPB-PGK-destination (Plasmid #60436).
    Depositor
    Yohei Hayashi
    Insert
    Venus-Akaluc
    Use
    Luciferase; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    209768-plasmid-map-sequence-id-417214

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  7. pSRKBB-empty

    Plasmid
    #59449
    Purpose
    Contains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.
    Depositor
    Claudia Schmidt-dannert
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    modified lac
    Available since
    Sept. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    59449-plasmid-map-sequence-id-92571

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  8. pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid
    #215010
    Purpose
    Express a Mitophagy reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    pSu9 (Presequence of Fo-ATPase subunit 9)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215010-plasmid-map-sequence-id-429275

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  9. p416-GPD-HA-PPK

    Plasmid
    #183943
    Purpose
    Expresses HA-tagged E. coli PPK from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    PPK (ppk E. coli)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    WT
    Promoter
    GPD
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    183943-plasmid-map-sequence-id-363305

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  10. pExp-MBP

    Plasmid
    #112568
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-MBP and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112568-plasmid-map-sequence-id-217224

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  11. pCLX-PGK-hCDK4

    Plasmid
    #114314
    Purpose
    Constitutive expression of the CDK4 protein
    Depositor
    Patrick Salmon
    Insert
    CDK4 (CDK4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114314-plasmid-map-sequence-id-218864

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  12. pET-53-RtcA

    Plasmid
    #107876
    Purpose
    Expresses E. coli RtcA in bacterial expression cells
    Depositor
    Jay Hesselberth
    Insert
    E. coli RtcA (rtcA Escherichia coli)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    107876-plasmid-map-sequence-id-361816

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  13. pCMV-ABE8e-NRCH

    Plasmid
    #165416
    Purpose
    Expresses the base editor ABE8e-NRCH in mammalian cells
    Depositor
    David Liu
    Insert
    ABE8e-NRCH
    Use
    Tags
    BP-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165416-plasmid-map-sequence-id-334022

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  14. pLIX403-ccdB-EBFP2

    Plasmid
    #158562
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    158562-plasmid-map-sequence-id-310105

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  15. pLV Rnf121-V5

    Plasmid
    #175130
    Purpose
    Lentiviral expression of mouse Rnf121-V5
    Depositor
    Larry Gerace
    Insert
    Rnf121 (Rnf121 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175130-plasmid-map-sequence-id-352014

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  16. AAV-syn-LifeAct-jGCaMP8m-WPRE

    Plasmid
    #186039
    Purpose
    AAV-mediated expression of LifeAct-jGCaMP8m under the synapsin promoter
    Depositor
    Loren Looger
    Insert
    LifeAct-jGCaMP8m
    Use
    AAV
    Tags
    LifeAct-6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin
    Available since
    July 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    186039-plasmid-map-sequence-id-368190

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  17. pCDNA3-hTLR3-VenusFlag

    Plasmid
    #197636
    Purpose
    Human CD283 protein expression in mammalian cells
    Depositor
    Haian Fu
    Insert
    TLR3 (TLR3 Human)
    Use
    Tags
    Venus-Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    197636-plasmid-map-sequence-id-392775

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  18. pRetroQ-Cre-ERT2

    Plasmid
    #59701
    Purpose
    to make Cre-ERT2 expressing stable cell line by retrovirus
    Depositor
    Richard Youle
    Insert
    Cre-ERT2
    Use
    Retroviral
    Tags
    Estrogen receptor (ERT)
    Expression
    Mammalian
    Mutation
    Promoter
    cmvIE
    Available since
    Sept. 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    59701-plasmid-map-sequence-id-93474

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  19. pACYC-GST

    Plasmid
    #62329
    Purpose
    E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Glutathione S-transferase (GST) tag and TEV prote…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lac0
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62329-plasmid-map-sequence-id-296399

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  20. pFloxed DHFR-TS*

    Plasmid
    #100605
    Purpose
    pUC19 with loxP-pDHFR-TS:DHFR-TS*(Pyr-R)-Stop-loxP-DHFR-TS 3' UTR
    Depositor
    David Sibley
    Insert
    Floxed TgDHFR-TS*
    Use
    Toxoplasma expression
    Tags
    Expression
    Mutation
    Pyrimethamine resistance
    Promoter
    Available since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    100605-plasmid-map-sequence-id-192861

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Showing: 9661 - 9680 of 51990 results