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FUW TetO HA-AMPKa2 WT

Plasmid

#69826

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A

Plasmid

#182049

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S248A/T258A

Promoter

CAG

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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Flag‐mTim3 (T1 truncation)

Plasmid

#49210

Purpose

expresses Flag tagged mouse Tim3 (T1 truncation)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 T1 truncation (Havcr2 Mouse)

Use

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

T1 truncation ( contains all but the three most C…

Promoter

EF1a

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SEMA3D-COMP5AP-AviTag-9xHis

Plasmid

#157530

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA3D (SEMA3D Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-SaPE2-P2A-BSD

Plasmid

#174818

Purpose

Mammalian expression of SaCas9 prime editor 2 with P2A-BSD marker

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

SaPE2-P2A-BSD

Use

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Flag‐mTim3 (del.Cyto)

Plasmid

#49209

Purpose

expresses Flag tagged mouse Tim3 fragment (no cytosolic domain)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 (del.Cyto) (Havcr2 Mouse)

Use

Tags

CD8 signal sequence and FLAG

Expression

Mammalian

Mutation

cytosolic domain deleted, no Tim3 signal sequence…

Promoter

Ef1a

Available since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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Flag‐mTim3 (T2 truncation)

Plasmid

#49211

Purpose

expresses Flag tagged mouse Tim3 (T2 truncation)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 T2 truncation (Havcr2 Mouse)

Use

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

T2 truncation ( lacks all but one tyrosine, which…

Promoter

EF1a

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pSMPUW-miR-124-GFP-Puro

Plasmid

#117321

Purpose

Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

hsa-miR-124-1 (MIR124-1 Human)

Use

Lentiviral

Tags

GFP-Puro

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-P2A-BSD

Plasmid

#174819

Purpose

Mammalian expression of SpCas9 prime editor 2 with P2A-BSD marker

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PE2-P2A-BSD

Use

Tags

P2A-BSD and SV40 bpNLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-tetpA(hBCL2-miR9/9*-124)-iRPT

Plasmid

#154881

Purpose

Doxycycline-dependently expresses human BCL2 and murine miR9/9*-124. Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.

Depositor

Hideyuki Okano

Article

Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

Inserts

iRFP713
Puromycin resistance gene
rtTAM2
BCL2 (BCL2 Human)
miR9/9*-124

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pIS1 INSIG2

Plasmid

#60798

Purpose

Luciferase reporter to measure miR-155 mediated differential repression. Contains INSIG2 3' UTR and wild-type miR-155 sites

Depositor

David Bartel

Article

Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

Insert

INSIG2 3'UTR and wild-type miR-155 binding site (INSIG2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

thymidine kinase (HSV-TK promoter)

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pIS1 mINSIG2

Plasmid

#60799

Purpose

Luciferase reporter to measure miR-155 mediated differential repression. Contains INSIG2 3' UTR and mutated miR-155 sites

Depositor

David Bartel

Article

Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

Insert

INSIG2 3'UTR and mutated miR-155 binding site (INSIG2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

mutated miR-155 binding site

Promoter

thymidine kinase (HSV-TK promoter)

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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DNA-CARzeta-GFP

Plasmid

#89344

Purpose

2nd generation lentiviral vector which expresses DNA-CAR receptor fused to GFP

Depositor

Ron Vale

Article

Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

Insert

DNA-CARzeta (CD247, CD86 Human)

Use

Lentiviral

Tags

SNAPf tag and mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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HcKCR2_mCherry_pcDNA3.1

Plasmid

#177337

Purpose

Expresses KCR2 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

Insert

HcKCR2

Use

Tags

mCherry

Expression

Mammalian

Mutation

mammalian codon-adapted

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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Teto Ngn2

Plasmid

#62223

Purpose

Inducible expression of Ngn2

Depositor

Kristin Baldwin

Article

Blanchard et al Nat Neurosci. 2015 Jan;18(1):25-35. doi:

Insert

Neurog2 (Neurog2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV-Myc-Caspase-4_W267N

Plasmid

#214311

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

W267N

Promoter

MESV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pOIMars1CyN

Plasmid

#75221

Purpose

Mammalian cell surface expression of fluorogen activating protein Mars1Cy

Depositor

Alan Waggoner

Article

Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

Insert

ss-HA-Mars1Cy-CD80(237-306)

Use

Tags

HA epitope

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_PTS2

Plasmid

#65676

Purpose

Expresses PTS2-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 2

Use

Luciferase

Tags

CNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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pONL-N1_PTS2

Plasmid

#65677

Purpose

Expresses PTS2-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

peroxisomal targeting signal 2

Use

Luciferase

Tags

ONL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D -IgK- TurboID- NCAN ELS-HA

Plasmid

#219809

Purpose

Secreted HA-tagged TurboID-NCAN-ELS domain for extracellular proximity labeling

Depositor

Cagla Eroglu

Article

Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

Insert

GfaABC1D

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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