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shGLDC-DOX

Plasmid

#72883

Purpose

dox inducible knockdown

Depositor

David Sabatini

Article

Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

Insert

RNAi hairpin against GLDC

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only
pLenti-3xFLAG-Puro PAK5 K478R K469R

Plasmid

#167180

Purpose

puromycin resistant lentiviral vector for expression of PAK5 K478R K469R

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 K478R K469R

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

UBC

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-617.sensor.PGK.dNGFR.WPRE

Plasmid

#85921

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-617 Sensor (MIR617 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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STK11 gRNA (BRDN0001147319)

Plasmid

#75914

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK11

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK11 (STK11 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK4 gRNA (BRDN0001147572)

Plasmid

#76178

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK4 (CDK4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Ngn1

Plasmid

#118589

Purpose

Expresses Ngn1 from the TRE3G promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Ngn1 (Neurog1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro

Plasmid

#50417

Purpose

Fluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.

Depositor

Andrew Scharenberg

Inserts

eGFP with I-Sce I TS
+3 mCherry

Use

Lentiviral

Tags

T2A dislinker and iRFP

Expression

Mammalian

Mutation

embedded I-Sce I TS from 163-185

Promoter

SFFV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-N-cG-Zeo

Plasmid

#124979

Purpose

Express Nanoluc, copGFP

Depositor

Kazuhiro Oka

Insert

Nanoluc, copGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1-alpha

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-OptoCAR-mScarlet

Plasmid

#170463

Purpose

Expresses an optically-controllable chimeric antigen receptor that is dependent on dimerizer drug, fused to mScarlet fluorophore

Depositor

John James

Article

Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

Insert

OptoCAR

Use

Lentiviral

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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hgRNA-A21_pLKO-Puro

Plasmid

#100570

Purpose

hgRNA-A21 in a lentiviral backbone with puromycin resistance

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

hgRNA-A21

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pInducer21-3xFlag-S208C-MEFV

Plasmid

#167019

Purpose

Lentiviral plasmid with 3xFlag-pyrin (S208C/PAAND variant) under the control of a doxycycline-inducible promoter

Depositor

Thomas Henry

Article

Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mutation

Serine 208 changed to Cysteine

Promoter

CMV Dox inducible

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shID2 #1

Plasmid

#83086

Purpose

Lentiviral shRNA vector for inducible knockdown of human ID2

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shID2 (ID2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLV-EF1a-V5-Vrk2

Plasmid

#120240

Purpose

Expresses V5-tagged Vrk2 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Vrk2 (Vrk2 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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SIK2 gRNA (BRDN0001148975)

Plasmid

#77139

Purpose

3rd generation lentiviral gRNA plasmid targeting human SIK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SIK2 (SIK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Txnip

Plasmid

#178437

Purpose

doxycycline-inducible expression of Mouse Txnip in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

Txnip (Txnip Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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ADSS_pLX307

Plasmid

#98316

Purpose

Lentiviral expression of ADSS

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

ADSS (ADSS2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLgw EcoDam-V5-SRF

Plasmid

#98602

Purpose

Mammalian DamID lentiviral vector forSRF with Dam-V5 using Gateway cloning

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

SRF (Srf Mouse)

Use

Lentiviral; Damid

Tags

Dam (DNA adenine methyltransferase) and V5

Expression

Mammalian

Mutation

Promoter

Heat Shock Minimal Promoter

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO_S.C.V5_NGFR

Plasmid

#158331

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

S.C.V5

Expression

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-AGM-VER9063 vpr

Plasmid

#115825

Purpose

Encodes codon optimized 3xFLAG-SIVAGM Vpr (Ver 9063) (L40990) and Puro resistance marker

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVAGM Vpr (Ver 9063)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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QQR1 variant

Plasmid

#138564

Purpose

Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

QQR1

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

G1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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