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pET15 PemrR-gfp biosensor

Plasmid

#185793

Purpose

The pET15 PemrR-gfp biosensor is transcriptional fusion of GFP with the promoter of the emrRAB operon. This biosensor is responsive to monoaromatics, such as vanillin and syringaldehyde.

Depositor

Steven Hallam

Article

Ho et al ACS Synth Biol. 2018 Feb 16;7(2):392-398

Insert

pEmrR-gfp

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

PemrR

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Plekhh1-MyTH-FERM

Plasmid

#187370

Purpose

Expresses human Plekhh1-MyTH-FERM labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1-MyTH-FERM

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-Alox12-PLAT-EGFP-6H

Plasmid

#187190

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000069463 (alox12 Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pET30-T7-lacO-His-sfGFP

Plasmid

#180754

Purpose

pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Use

Tags

strep tag

Expression

Bacterial

Mutation

G140C- Please see depositor comments

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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FUGW-UBC-EGFP-2A-dnVAMP2

Plasmid

#185723

Purpose

Lentiviral expression of GFP and dominant-negative VAMP2 from UbC promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

VAMP-2 (VAMP2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

deleted AA 97-116

Promoter

hUbC

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAG8H-GFP-EWS_1-120

Plasmid

#180464

Purpose

Expresses the construct His8-GFP-EWS_1-120 with a TEV cleavage site between GFP and EWS_1-120

Depositor

David Libich

Article

Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

Insert

EWSR1 (EWSR1 Human)

Use

Tags

GFP and His8

Expression

Bacterial

Mutation

deleted 121f-656

Promoter

T7

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFOX12-Tier1-PhCMV-eGFP

Plasmid

#169509

Purpose

Tier-1 vector encoding PhCMV-driven green fluorescent protein eGFP (PhCMV-eGFP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven enhanced green fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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NLS-tdPCP-GFP-VP16

Plasmid

#183935

Purpose

PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdPCP-GFP-VP16 (UL48 Synthetic)

Use

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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p8133 MSCV GFP Neo C-HA

Plasmid

#184522

Purpose

Expression of GFP

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

GFP

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

MSCV LTR

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf cdh1-deltaCyot-GFP

Plasmid

#185412

Purpose

GFP tagged zebrafish cdh1 lacking the cytoplasmic domain.

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

cdh1 (cdh1 Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Mutation

Cytoplasmic domain deleted

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO5-SgHottip-GFP-CRISPRi

Plasmid

#134989

Purpose

dCas9-mediated inactivation of HOTTIP in mammalian cells

Depositor

Suming Huang

Article

Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

Insert

HOTTIP (HOTTIP Human)

Use

Lentiviral

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40-A253FY208A_X

Plasmid

#147973

Purpose

Insect Expression of DmCaf40-A253FY208A

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-A253FY208A (Rcd-1 Fly)

Use

Tags

EGFP (A179V)

Expression

Insect

Mutation

Promoter

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP humanAPOBEC1 L173A/G227A

Plasmid

#167171

Purpose

Expresses human APOBEC1 L173A/G227A in mammalian cells

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

humanAPOBEC1 (APOBEC1 Human)

Use

Tags

Expression

Mammalian

Mutation

changed Leucine 173 to Alanine; changed Glycine 2…

Promoter

CMV promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(172-554)

Plasmid

#183664

Purpose

N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 172-554

Promoter

CMV

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso2-6xmut_U

Plasmid

#147732

Purpose

Mammalian Expression of HsNot1iso2-6xmut

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot1iso2-6xmut (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

4 silent and two non silent G917E and R2353Q muta…

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T-L36AL39A_T

Plasmid

#147604

Purpose

Insect Expression of Dm4E-T-L36AL39A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T-L36AL39A (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

Two non silent mutations (H628R and N659S) compar…

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCup-Y342AL347A_O

Plasmid

#147103

Purpose

Insect Expression of DmCup-Y342AL347A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCup-Y342AL347A (cup Fly)

Use

Tags

Expression

Insect

Mutation

Y342A, L347A, I350L, P418S, K1045N

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat-dsRNAres_J

Plasmid

#146692

Purpose

Insect Expression of DmHPat-dsRNAres

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat-dsRNAres (Patr-1 Fly)

Use

Tags

Expression

Insect

Mutation

two non silent, A479V, G568D and one silent mutat…

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(211-554)

Plasmid

#183665

Purpose

N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 211-554

Promoter

CMV

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pUG36-NES-EGFP-cPHx3

Plasmid

#183668

Purpose

PtdIns(3,4)P2 biosensor for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

PLEKHA1 (PLEKHA1 Human, Frog)

Use

Tags

X. laevis map2k1.L(32-44)-EGFP

Expression

Yeast

Mutation

Amino acids 169-329 (tandem trimer)

Promoter

MET25

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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