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Plasmid#125782Purposeconstitutive expression of a short-hairpin RNA targeting human WRNDepositorInsertshWRN2 (WRN Human)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Nras61K-Puro
Plasmid#49404PurposeMSCVpuro based vector, expressing Nras mutant 61K.DepositorInsertN Ras K61 (NRAS Human)
UseRetroviralTagsExpressionMammalianMutation61K constitutively activatedPromoterMSCV promotorAvailable sinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-sgNUAK2
Plasmid#138693PurposeExpresses a human NUAK2-targeting sgRNA and Cas9DepositorInsertsgNUAK2 human (NUAK2 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-WT
Plasmid#91986PurposeExpresses FLAG-HA-AGO2 (WT)DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW-ND-Cdt1-HA-Puro
Plasmid#193767PurposeDoxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal HA tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertND-Cdt1-HA (CDT1 Human)
UseLentiviralTagsHAExpressionMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-RalA-wt
Plasmid#15251DepositorInsertv-ral simian leukemia viral oncogene homolog A (RALA Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLX302 LacZ-V5 puro
Plasmid#98579PurposeLentiviral vector for constitutive expression of human LacZ with C-terminal V5 tagDepositorInsertLacZ
UseLentiviralTags2x V5 tagExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-SunTag-PuroR
Plasmid#80968PurposeCRISPaint gene taggingDepositorInsertSunTag v4 (1x)
UseGene taggingTagsExpressionMutationPromoternoneAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
H2B-2A-GFP-IRESpuro2
Plasmid#183746PurposeExpresses H2B-2A-GFPDepositorInsertH2B (H2BC16P Human)
UseTagsGFP with 2A protease cleavage site between H2B an…ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MXS_PGK::PuroR-bGHpA
Plasmid#62440PurposeMXS_chaining vector with PGK::PuroR-bGHpADepositorInsertresistance cassette against Puromycin with PGK Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFV*/Puro2a-GFP
Plasmid#198222PurposeLentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFPDepositorInsertEGFP
UseLentiviralTagsCodon-optimized puromycin resistance gene, follow…ExpressionMammalianMutationPromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
shERK1a-mlpx-puro
Plasmid#65228Purposeencodes a shRNA against ERK1DepositorInsertshRNA against ERK2 (MAPK1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Puro-TTL-wt-TTF-1
Plasmid#67657PurposeTo create a dox-on inducible expression of human wt-TTF-1 cDNA in cellsDepositorInsertThyroid Transcription Factor 1 (NKX2-1 Human)
UseRetroviralTagsN/AExpressionMutationPromoterAvailable sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRE-T2-miR-PURO
Plasmid#102646PurposeTet inducible microRNA and cDNA expressionDepositorTypeEmpty backboneUseRNAiTagsExpressionMammalianMutationPromoterTet/CMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-sgMARK2
Plasmid#138689PurposeExpresses a human MARK2-targeting sgRNA and Cas9DepositorInsertsgMARK2 human (MARK2 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-sgMARK3
Plasmid#138690PurposeExpresses a human MARK3-targeting sgRNA and Cas9DepositorInsertsgMARK3 human (MARK3 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-dKeNLuc_SV40:PuroR
Plasmid#183046PurposeExpresses the FLAG-tagged dKeNLuc (Far-Red dKeima LumiFluor) in mammalian cells.DepositorInsertdKeNLuc LumiFluor
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-KaNLuc_SV40:PuroR
Plasmid#183047PurposeExpresses the FLAG-tagged KaNLuc (Far-Red LSSmKate2 LumiFluor) in mammalian cells.DepositorInsertKaNLuc LumiFluor
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-StrepTag II-PuroR
Plasmid#80967PurposeCRISPaint gene taggingDepositorInsertStrepTag II
UseGene taggingTagsExpressionMutationPromoternoneAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3PA-Puro-iTk
Plasmid#109067Purposeinserts three polyA sites to end transcription of a targetted gene and a puromycin cassette for selectionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEFSAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only