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Plasmid#118041PurposeGABAA receptor expression (gamma3 subunit) Myc-tag near N-terminusDepositorInsertGABRG3 (Gabrg3 Rat)
UseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma1 (in pCDNA3.1)
Plasmid#118038PurposeGABAA receptor expression (gamma1 subunit) Myc-tag near N-terminusDepositorInsertGABRG1 (Gabrg1 Rat)
UseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-3xHA-mASL
Plasmid#34577PurposeNOTE: This plasmid contains 1xHA-mASL, not 3xHA-mASL.DepositorInsertASL (Asl Mouse)
UseTags1xHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-FbxO10-pcDNA3.1-
Plasmid#52508Purposeexpresses human FbxO10 in mammalian cells with FLAG-HA tag at N-terminusDepositorInsertFbxO10 (FBXO10 Human)
UseTagsFLAG-HAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nluc(W12stop)
Plasmid#180502PurposeMammalian expression of Nluc(W12stop)DepositorInsertNluc(W12stop)
UseTagsFLAGExpressionMammalianMutationW12(UGG) to amber stop codon(UAG)PromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) EPHB4 Sense
Plasmid#60638PurposeExpresses the human EPHB4 exon 11/12 circular RNADepositorInsertEphrin type B receptor 4 (EPHB4 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)MBII-85-5'/3'ss mut
Plasmid#67645PurposeMBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.DepositorInsertMBII85 snoRNA
UseTagsExpressionMammalianMutation5' and 3' splice sites mutationsPromoterAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pY112 (pcDNA3.1-huSaCpf1)
Plasmid#92269PurposeExpression of humanized SaCpf1DepositorInserthuSaCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY114 (pcDNA3.1-huPgCpf1)
Plasmid#92273PurposeExpression of humanized PgCpf1DepositorInserthuPgCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY122 (pcDNA3.1-huCPbCpf1)
Plasmid#92298PurposeExpression of humanized CPbCpf1DepositorInserthuCPbCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY118 (pcDNA3.1-huLb4Cpf1)
Plasmid#92294PurposeExpression of humanized Lb4Cpf1DepositorInserthuLb4Cpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CONK1-lumitoxin
Plasmid#51689PurposeExpression vector for CONK1-lumitoxinDepositorInsertCONK1-lumitoxin
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-EKAR
Plasmid#181858PurposeGenetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-EKAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag-Sesn1b
Plasmid#61867PurposeTransient overexpression of Flag-Sesn1b (mouse)DepositorInsertSesn1 (Sesn1 Mouse)
UseTagsFlagExpressionMammalianMutationnonePromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 (-) hYAP 1-1alpha
Plasmid#124139PurposeProtein expression for funtional studies of cell growth promotionDepositorInsertYAP1 (YAP1 Human)
UseUnspecifiedTagsExpressionMutationNone (Wild Type)PromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ERBB4 (R544W)
Plasmid#29531DepositorInsertERBB4 (ERBB4 Human)
UseTagsExpressionMammalianMutationchanged arginine 544 to tryptophanPromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/3xmyc_NLRP11-NACHT
Plasmid#131199Purposemammalian expressionDepositorInsertNACHT of NLRP11 (NLRP11 Human)
UseTagsmycExpressionMammalianMutationcontains only the NACHT domain (amino acids 125-5…PromoterpCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CCND1(T286A)
Plasmid#172631PurposeExpresses untagged cyclin D1(T286A) in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseTagsExpressionMammalianMutationThr286AlaPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 AT1AR-324delta
Plasmid#45635DepositorInsertAT1AR-324delta (Agtr1a Rat)
UseTagsHAExpressionMammalianMutationadded stop codon at 324 aaPromoterCMVAvailable sinceAug. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nAChr-beta3
Plasmid#48811PurposeThis report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.DepositorInsertnAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
UseTagsExpressionMammalianMutation*PromoterCMVAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only