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Showing: 2351 - 2400 of 3976 results

  4. IL1RN gRNA1

    Plasmid
    #113130
    Purpose
    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for gRNA expression, RSV for GFP expression
    Available since
    March 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    113130-plasmid-map-sequence-id-217998

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  7. pSLIK-Venus-TmiR-G13-G12

    Plasmid
    #25742
    Purpose
    Lentiviral vector with Tet-based inducible expression of both mouse G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus fluorescent protein coexpression.
    Depositor
    Iain Fraser
    Article
    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1
    Insert
    G alpha 13 and 12 miR-shRNA
    Use
    Lentiviral and RNAi
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 14, 2010
    Availability
    Academic Institutions and Nonprofits only
    25742-plasmid-map-sequence-id-12067

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  21. pCVL.SSA TLR (Ani)

    Plasmid
    #45577
    Purpose
    Codes for the SSA-TLR with the target site for I-Ani I in a lentiviral backbone
    Depositor
    Andrew Scharenberg
    Article
    Kuhar et al Nucleic Acids Res. 2013 Oct 10.
    Inserts
    5' iRFP arm
    eGFP with I-Ani I TS
    +3 mCherry
    3' iRFP arm
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    embedded I-Ani I TS from 163-185bp, truncated 25 …
    Promoter
    SFFV
    Available since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    45577-plasmid-map-sequence-id-67848

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  28. pLenti_EF1α-NES-Kinprola_on-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid
    #233373
    Purpose
    EF1α driven co-expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP and Cas9 expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Inserts
    NES-Kinprola_on-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    Use
    Lentiviral
    Tags
    FLAG, NES, and mEGFP
    Expression
    Mutation
    Promoter
    EF1α
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233373-plasmid-map-sequence-id-466778

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  29. pLenti_EF1α-NES-Kinprola_PKA-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid
    #233371
    Purpose
    EF1α driven co-expression of the PKA activity recorder Kinprola_PKA fused to mEGFP and Cas9 expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Inserts
    NES-Kinprola_PKA-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    Use
    Lentiviral
    Tags
    FLAG, NES, and mEGFP
    Expression
    Mutation
    Promoter
    EF1α
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233371-plasmid-map-sequence-id-466776

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  30. pLenti_EF1α-NES-Kinprola_PKA_T/A-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid
    #233372
    Purpose
    EF1α driven co-expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A fused to mEGFP and Cas9 expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Inserts
    NES-Kinprola_PKA_T/A-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    Use
    Lentiviral
    Tags
    FLAG, NES, and mEGFP
    Expression
    Mutation
    Promoter
    EF1α
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233372-plasmid-map-sequence-id-466777

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  31. pEND-224_Cacnes_sfGFP

    Plasmid
    #225611
    Purpose
    Replicative vector for green fluorescent reporter protein sfGFP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+sfGFP.
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    sfGFP
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225611-plasmid-map-sequence-id-451364

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  32. pEND-225_Cacnes_mKO2

    Plasmid
    #225612
    Purpose
    Replicative vector for orange fluorescent reporter protein mKO2 strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mKO2
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    mKO2
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225612-plasmid-map-sequence-id-451369

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  42. pBIOS1458

    Plasmid
    #171715
    Purpose
    expresses maize Glutamine Synthetase ZmGln1-3 cDNA under the control of the Cassava Vein Mosaic Virus promoter (CsVMV) (cassette 1) and the maize Rubisco small subunit promoter (ZmRbcS) (cassette 2)
    Depositor
    Christophe Sallaud
    Article
    Amiour et al Commun Biol. 2021 Sep 17;4(1):1095. doi:
    Insert
    ZmGln1-3
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    171715-plasmid-map-sequence-id-378134

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  43. pC1_oROS-HT

    Plasmid
    #216413
    Purpose
    Expresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.
    Depositor
    Andre Berndt
    Article
    Lee et al bioRxiv 2024.02.06.579232
    Insert
    oROS-HT
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216413-plasmid-map-sequence-id-435275

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  44. pC3.1_CMV_oROS-G_LF(C199S)

    Plasmid
    #216112
    Purpose
    Expresses the loss-of-function mutations C199S of the genetically encoded green fluorescent hydrogen peroxide sensor oROS-G in mamalian cells.
    Depositor
    Andre Berndt
    Article
    Lee et al bioRxiv 2024.01.31.578117
    Insert
    oROS-G_LF(C199S)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216112-plasmid-map-sequence-id-434850

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  46. pAN057

    Plasmid
    #220053
    Purpose
    NMD-reporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (c.3846G>A mutation; W1282X).
    Depositor
    Blake Wiedenheft
    Article
    Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1
    Insert
    fLuc-CFTR(W1282X, exons 22-27) (CFTR Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    c.3846G>A mutation
    Promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220053-plasmid-map-sequence-id-434400

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  48. AAV2_CAG_oROS-G_LF(C199S)

    Plasmid
    #216116
    Purpose
    Encodes the loss-of-function mutation C199S of the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.
    Depositor
    Andre Berndt
    Article
    Lee et al bioRxiv 2024.01.31.578117
    Insert
    oROS-G_LF(C199S)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216116-plasmid-map-sequence-id-429714

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  50. P2061

    Plasmid
    #186299
    Purpose
    Expresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoter
    Depositor
    Joerg Stelling
    Article
    Azizoglu et al Curr Protoc. 2023 Jan;3(1):e647. doi: 10
    Inserts
    LexA-HBD-B112
    Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    2xLexop-minimalCyc1 and PACT1
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    186299-plasmid-map-sequence-id-367190

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Showing: 2351 - 2400 of 3976 results