Addgene

We narrowed to 2,243 results for: hells

Showing: 1411 - 1440 of 2243 results

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pET28a D315A

Plasmid

#139592

Purpose

expresses SznF D315A

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF D315A

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

changed apartate 315 to alanine

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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flk1 promoter

Plasmid

#26436

Depositor

Didier Stainier

Article

Jin et al Development. 2005 Dec . 132(23):5199-209

Insert

flk1 promoter

Use

Topo cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pET28a-SznE

Plasmid

#139282

Purpose

expresses SznE in E. coli

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

sznE

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCLEN

Plasmid

#17704

Depositor

Nicholas Gaiano

Article

Gaiano et al Neuron. 2000 May . 26(2):395-404.

Insert

NIC (Notch1 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2008

Availability

Academic Institutions and Nonprofits only
pET28a SznF E281A

Plasmid

#139591

Purpose

expresses SznF E281A

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF E281A

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

changed glutamate 281 to alanine

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a SznF H225A

Plasmid

#139590

Purpose

expresses SznF H225A

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF H225A

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

changed histidine 225 to alanine

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-SznF E215A

Plasmid

#139565

Purpose

expresses SznF E215A in E. coli

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF E215A

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

changed glutamate 215 to alanine

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-SznF

Plasmid

#139564

Purpose

expresses SznF in E. coli

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSANG10-3F

Plasmid

#39264

Depositor

John McCafferty

Article

Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

Type

Empty backbone

Use

Bacterial expression, bacterial expression of re…

Tags

6xHis, pelB leader, and tri-FLAG

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2+ centrin-RFP

Plasmid

#26753

Depositor

Christopher Kintner

Article

Park et al Nat Genet. 2008 Jul . 40(7):871-9.

Insert

Centrin 4 (cetn4 Frog)

Use

Reporter for basal body

Tags

monomeric RFP

Expression

Mutation

Promoter

Available since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pEF-I-GFP GX

Plasmid

#45443

Depositor

John Brigande

Article

Gubbels et al Nature. 2008 Sep 25. 455(7212):537-41.

Type

Empty backbone

Use

Tags

IRES EGFP

Expression

Mammalian

Mutation

Promoter

human EF1α

Available since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+ tecktin-GFP

Plasmid

#26932

Depositor

Christopher Kintner

Article

Park et al Nat Genet. 2008 Jul . 40(7):871-9.

Insert

Tektin 2 (tekt2 Frog)

Use

Tags

GFP

Expression

Mammalian

Mutation

I429A

Promoter

Available since

Dec. 14, 2012

Availability

Academic Institutions and Nonprofits only
pBALU-ext

Plasmid

#69339

Purpose

It allows to extend the genomic insert in a fosmid.

Depositor

Oliver Hobert

Article

Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

Insert

kanamycin gene +pCC1FOS homology

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only
pRS414-59

Plasmid

#23087

Depositor

Sharon Dent

Article

Edmondson et al J Biol Chem. 2002 Aug 16. 277(33):29496-

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H3 changed Serine 10 to Alanine, H3 S10A.

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pMCV

Plasmid

#199743

Purpose

Minimal cloning vector

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pANG03

Plasmid

#178085

Purpose

Tetracycline resistant backbone for in vivo Gateway reaction. Plasmid contains gateway cassette with restriction sites to add promoters or C and N Terminal tags. Compatible with pANG02.

Depositor

Sören Abel

Article

Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

NA

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pANG01

Plasmid

#178083

Purpose

Carbenicillin resistant backbone for in vivo Gateway reaction. Plasmid contains gateway cassette with restriction sites to add promoters or C and N Terminal tags. Compatible with pANG02.

Depositor

Sören Abel

Article

Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

NA

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-SznF H407A H409A H448A

Plasmid

#139566

Purpose

expressed SznF H407A H409A H448A in E. coli

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF H407A H409A H448A

Use

Tags

hexahistidine

Expression

Bacterial

Mutation

changed glutamate 215 to alanine

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJD112

Plasmid

#23088

Depositor

Sharon Dent

Article

Edmondson et al J Biol Chem. 2002 Aug 16. 277(33):29496-

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone 3 Changed Serine 10 to Aspartate, H3 S10D.

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pJD113

Plasmid

#23089

Depositor

Sharon Dent

Article

Edmondson et al J Biol Chem. 2002 Aug 16. 277(33):29496-

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H3 changed Serine 10 to Glutamamte, H3 S1…

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pGFP-Rab8A

Plasmid

#24898

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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LV-H2B-mRFP1

Plasmid

#47376

Purpose

This was used as a nuclear marker

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

H2B

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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PCx35IL-pET42

Plasmid

#42916

Depositor

John O'Brien

Article

Burr et al Biochem Biophys Res Commun. 2005 Oct 7;3

Insert

Connexin 35 cytoplasmic loop

Use

Tags

6x His, GST, and S-tag

Expression

Bacterial

Mutation

cytoplasmic loop is aa 101-179

Promoter

T7

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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Vh-HA-DHFR

Plasmid

#20217

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

DHFR (folA E. coli)

Use

Tags

HA and Vh

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
Vh-HA-3xDHFR

Plasmid

#20219

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xDHFR (folA E. coli)

Use

Tags

HA and Vh

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pGL3-sbIL6P

Plasmid

#65099

Purpose

Luciferase reporter construct under the seabream IL6 promoter.

Depositor

Josep Planas

Article

Castellana et al Fish Shellfish Immunol. 2013 Jul;35(1):7

Insert

interleukin-6 promoter

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only
pENTR-fishRab8A

Plasmid

#24904

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

fishRab8A

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

June 28, 2010

Availability

Academic Institutions and Nonprofits only
pENTR-fishRab8B

Plasmid

#24907

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

fishRab8B

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

June 28, 2010

Availability

Academic Institutions and Nonprofits only
Vh-HA-2xDHFR

Plasmid

#20218

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

2xDHFR (folA E. coli)

Use

Tags

HA and Vh

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Zeo-ssIFNA2

Plasmid

#183469

Purpose

Expression of the Salmo salar interferon ifna2

Depositor

Bertrand Collet

Article

Collins et al Fish Shellfish Immunol. 2014 Oct;40(2):6

Insert

Salmo salar interferon alpha 2 precursor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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