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Plasmid#141543PurposeLentiviral vector for overexpressing the RELA transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRELA (RELA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
35S:YFPc-RabG3b-DN
Plasmid#102374Purposefluorescent tagging. Plant expression of YFPc-RabG3b-DNDepositorInsertRabG3B (RABG3B Mustard Weed)
UseTagsYFPcExpressionPlantMutationT22NPromoter35SAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
35S:YFPc-RabG3b-WT
Plasmid#102373Purposefluorescent tagging. Plant expression of YFPc-RabG3b-WTDepositorInsertRabG3B (RABG3B Mustard Weed)
UseTagsYFPcExpressionPlantMutationPromoter35SAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pLVX-SRRM2-mCherry
Plasmid#174089PurposeInducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on systemDepositorInsertSRRM2 (SRRM2 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterTREtightAvailable sinceSept. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-hMLH1dn
Plasmid#174828PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)DepositorInsertPEmax-P2A-hMLH1dn
UseTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDetailed in manuscript; deletion of residues 754-…PromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-TBG-FLAG-mRIPK1
Plasmid#115341PurposeLiver-specific adeno-associated viral delivery and mammalian expression of Flag-mRIPK1DepositorInsertRIPK1 (Ripk1 Mouse)
UseAAVTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
ALIX-mNeonGreen
Plasmid#191191PurposeFull-length ALIX with C-terminal mNeonGreen tag.DepositorInsertALIX (PDCD6IP Synthetic, Human)
UseTags6xHIS and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag mTOR wt
Plasmid#26603PurposeMammalian expression of human flag-tagged mTorDepositorInsertmTOR (MTOR Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBMN(CMV-copGFP-Luc2-Puro)
Plasmid#80389PurposeTo make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection markerDepositorInsertcopGFP-T2A-Luc2-F2A-Puromycin
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHBS1389 IBB-GFP-mCherry3E
Plasmid#118803PurposeFluorescence-based TDP43-dependent splicing reporterDepositorInsertSynthetic
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST JNKKTRClover
Plasmid#59151PurposeLentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertJNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-GFP11-Clathrin light chain
Plasmid#70217PurposeExpresses GFP11-clathrin light chain in mammalian cellsDepositorInsertGFP11-Clathrin light chain (CLTA Human)
UseTagsGFP11ExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST p38KTRClover
Plasmid#59152PurposeLentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertp38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(Caspase-1 cleavage seq) T2A mCherry
Plasmid#124494PurposeExpresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherryDepositorInsertFlipGFP(Caspase-1 cleavage seq) T2A mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
ADRB2-Tango
Plasmid#66220PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertADRB2 (ADRB2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEcCas
Plasmid#73227PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRNADepositorInsertsCas9
sgRNA
UseCRISPR; Red recombinaseTagsExpressionBacterialMutationPromoternative promoterAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-TIS11B
Plasmid#121157PurposeMammalian expression of GFP tagged TIS11BDepositorInsertZFP36L1 (TIS11B) (ZFP36L1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_ssSBP-EGFP
Plasmid#65277Purposeto synchronize transport of secretory soluble GFP from the ER (RUSH system)DepositorInsertEGFP
UseTagsIL-2 signal sequence and Streptavidin Binding Pr…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b-RvLEAMshort
Plasmid#218122PurposeExpresses truncated RvLEAM protein under IPTG induction in bacterial host cells.DepositorInsertRvLEAMshort
UseTags6xHISExpressionBacterialMutationTruncated form (58-181aa)PromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only