Showing: 9541 - 9560 of 16040 results
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Plasmid#68822PurposeEGFP tagged Collybistin expression in mammalian cellsDepositorInsertCollybistin (Arhgef9 Rat)
UseTagsEGFPExpressionMammalianMutationnonePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-IKZF3-3xHA-pGK-Pur
Plasmid#69053Purposelentiviral expression of IKZF3 with HA tagDepositorInsertIKZF3 (IKZF3 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterUBCAvailabilityAcademic Institutions and Nonprofits only -
ch-TOGKDP-GFP
Plasmid#69112PurposeFor mammalian expression of knockdown-proof human ch-TOG tagged with EGFP.DepositorInsertColonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
UseTagsEGFPExpressionMammalianMutationSilent mutations to confer shRNA resistance.PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
cpVenus-Gγ2
Plasmid#69626PurposeThe heterotrimeric G-protein subunit, Gγ2, tagged with circular permutated Venus (cp173V).DepositorInsertcp173Venus-Ggamma2 (GNG2 Synthetic)
UseTagsciruclar permutated Venus (cp173V)ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-HA-MEK1 C121S
Plasmid#53197PurposeExpresses HA tagged human MEK1 C121S from puromycin resistance retroviral vectorDepositorInsertMEK1 (MAP2K1 Human)
UseRetroviralTagsHAExpressionMammalianMutationC121SPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-HA-MEK5 CBM-E
Plasmid#53199PurposeExpresses HA tagged human MEK5 L280A/H281A/M323A/Q332A from puromycin resistance retroviral vectorDepositorInsertMEK5 (MAP2K5 Human)
UseRetroviralTagsHAExpressionMammalianMutationL280A/H281A/M323A/Q332APromoterAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-YOD1-MutS2-C160S
Plasmid#85668PurposeExpression of human YOD1 I292Q V295Q C160S mutant with N-terminal GFP tagDepositorInsertYOD1 (YOD1 Human)
UseTagsGFPExpressionMammalianMutationC160S and I292Q and V295QPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pKCAG-H2BmCherry-OptoJNKi
Plasmid#89741PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11DepositorInsertOptoJNKi
UseOptogeneticsTagsHistone2B and mCherryExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
HOXB6 (human) FLAG pCMV
Plasmid#8815DepositorInsertHOXB6 (HOXB6 Human)
UseTagsFLAGExpressionMammalianMutationcontains improved ribosomal binding site and FLAG…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pAC94-pmax-dCas9VP160-2A-puro
Plasmid#48226PurposedCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.DepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
UseCRISPRTags2A-puro, HA Tag, and VP160ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailabilityAcademic Institutions and Nonprofits only -
pAC95-pmax-dCas9VP160-2A-neo
Plasmid#48227PurposedCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.DepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
UseCRISPRTags2A-neo, HA Tag, and VP160ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailabilityAcademic Institutions and Nonprofits only -
pDEST-mCherry-GJA1-20k-N1
Plasmid#49861PurposeEncodes C-terminal fragment of human connexin 43 initiating at M213 with a V5 C-terminal fusion tagDepositorInsertGJA1-20k (GJA1 Human)
UseTagsmCherryExpressionMammalianMutationGJA1-20k corresponds to residues 213 to 382 to Co…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
YFP-Parkin C431N
Plasmid#46924Purposeexpresses mutant Parkin with YFP tag in mammalian cellsDepositorInsertPark2 (PRKN Human)
UseTagsEYFPExpressionMammalianMutationchanged cysteine 431 to asparaginePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pEB1N-LZ-LOV2(wt)
Plasmid#107614PurposeN-terminal half of π-EB1 with wild-type LOV2, no fluorescent tagDepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain and GCN4 leuc…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
ET8-GPIba-6His
Plasmid#102878PurposeWild type human platelet membrane protein GPIb alpha (1-290aa) with 6Histag at the C-terminusDepositorInsertHuman platelet membrane protein GPIb alpha 1-290aa (GP1BA Human)
UseTags6xHis tagExpressionMammalianMutationPromoterCMV promoterAvailabilityAcademic Institutions and Nonprofits only -
pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor
Plasmid#92142PurposeTargeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). Auxin-inducible degron system.DepositorInsertosTir1
UseMouse TargetingTagsV5 tagExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMDC43-FIT2
Plasmid#96993PurposeExpress GFP-tagged mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsGFPExpressionPlantMutationE2D (please see depositor comments below)Promoter35SAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-β-catenin_S23A,T40A,T41A,T112A
Plasmid#190824PurposeFor mammalian expression of β-catenin with 4 O-GlcNAc sites mutated (S23A, T40A, T41A and T112A) and GFP-tag on its N-terminus.DepositorInsertβ-catenin (CTNNB1 Chicken)
UseTagsGFPExpressionMammalianMutationS23A, T40A, T41A, T112APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pUGP1.1
Plasmid#196615PurposePlasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeastDepositorInsertUGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
UseTagsAID(71-114) and mCherryExpressionYeastMutationPromoterNative promoterAvailabilityAcademic Institutions and Nonprofits only -
pEB1N-mScarlet-LZ-LOV2
Plasmid#197419PurposeMammalian expression of the mScarlet-tagged N-terminal half of photo-inactivated π-EB1DepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain, GCN4 leucine…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 9541 - 9560 of 16040 results