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Addgene

pMDC43-FIT2
(Plasmid #96993)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 96993 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMDC43
  • Backbone size w/o insert (bp) 12460
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MmFIT2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    789
  • Mutation
    E2D (please see depositor comments below)
  • Entrez Gene
    Fitm2 (a.k.a. D930001I22Rik, Fit2)
  • Promoter 35S
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site PacI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sequences and maps of pMDC vectors can be obtained from the following website. http://www.botinst.uzh.ch/en/research/development/grossnik/vectors/MarkdGatewayVectors.html

Please note that mutation E2D was found during Addgene's quality control process. The depositor noted that this mutation does NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMDC43-FIT2 was a gift from Kent Chapman (Addgene plasmid # 96993 ; http://n2t.net/addgene:96993 ; RRID:Addgene_96993)
  • For your References section:

    Mouse fat storage-inducing transmembrane protein 2 (FIT2) promotes lipid droplet accumulation in plants. Cai Y, McClinchie E, Price A, Nguyen TN, Gidda SK, Watt SC, Yurchenko O, Park S, Sturtevant D, Mullen RT, Dyer JM, Chapman KD. Plant Biotechnol J. 2016 Dec 17. doi: 10.1111/pbi.12678. 10.1111/pbi.12678 PubMed 27987528