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TFORF2155

Plasmid

#141948

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

BCL11A (BCL11A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTM1_NP_ZEBOV

Plasmid

#69121

Purpose

Encodes for Ebola Virus Zaire NP under control of a T7 RNA polymerase promoter

Depositor

Elke Mühlberger

Article

Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

Insert

NP ZEBOV (NP )

Use

T7

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pFUGW-H1 empty vector

Plasmid

#25870

Purpose

3rd generation lentiviral vector

Depositor

Sally Temple

Article

Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pRS303-PADH1-AFB2

Plasmid

#99530

Purpose

S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, HIS3 marker (for use with the AID degron system)

Depositor

Helle Ulrich

Insert

AFB2 (AFB2 Mustard Weed)

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-Sox2

Plasmid

#13367

Purpose

Retroviral expression of mouse Sox2

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

SRY-box containing gene 2 (Sox2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pCAG-GCaMP6s

Plasmid

#171156

Purpose

Encodes GCaMP6s

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

GCaMP6s

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAG1247 H2B-ECFP-FIREmate

Plasmid

#205171

Purpose

Expresses H2B-ECFP-FIREmate in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

H2B-ECFP-FIREmate

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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9ao-SOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)

Plasmid

#209293

Purpose

mitoBE expression vector, target mtND4 gene.

Depositor

Wensheng Wei

Article

Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

Insert

SOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-SREBP-2

Plasmid

#26807

Depositor

Timothy Osborne

Article

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Insert

Sterol Regulatory Element Binding Protein 2 (SREBF2 Human)

Use

Tags

2x FLAG

Expression

Mammalian

Mutation

L7V, G76S, K395R and I482L

Promoter

CMV

Available since

July 26, 2011

Availability

Academic Institutions and Nonprofits only

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pTP288

Plasmid

#113460

Purpose

human Flag tagged Nbs1 in baculovirus transfer vector

Depositor

Tanya Paull

Article

Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

Insert

Nbs1 (NBN Human)

Use

Tags

Flag

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pClneoMyc mouse LNX2

Plasmid

#37010

Depositor

Yutaka Hata

Article

Kansaku et al Oncogene. 2006 Aug 24;25(37):5071-84. Ep

Insert

LNX2 (Lnx2 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2012

Availability

Academic Institutions and Nonprofits only
LeGO-T2

Plasmid

#27342

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, tdTomato

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-EGFP-T2A-Bsd

Plasmid

#193198

Purpose

Lentiviral vector expressing EGFP (driven by EFS promoter) and a blasticidin resistance marker

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Pur-mEGFP

Plasmid

#186329

Purpose

Fluorescent reporter system

Depositor

Bertrand Collet

Insert

mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAX APP 751

Plasmid

#30138

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein (APP Human)

Use

Tags

Expression

Mammalian

Mutation

wild type splice variant 751

Promoter

Available since

Aug. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pNICKclos1.0

Plasmid

#73639

Purpose

Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

Depositor

Sheng Yang

Article

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Inserts

Cas9 nickase
sGRNA to pyrE

Use

E.coli-clostridium shuttle vector

Tags

Expression

Mutation

D10A

Promoter

j23119 and ptb

Available since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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pUA66 theta GFP

Plasmid

#105607

Purpose

Expresses GFP with no promoter

Depositor

Pamela Silver

Article

Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

No Promoter

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pQE-60NA-msGFP2

Plasmid

#135301

Purpose

Encodes a photostable monomeric superfolder GFP

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

msGFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C9orf72

Plasmid

#83437

Purpose

Expresses GFP-C9orf72 in mammalian cells

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

C9orf72 (C9orf72 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pdCas9-Tet1-CD

Plasmid

#83340

Purpose

To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

Depositor

Ronggui Hu

Article

Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

Insert

dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

U6, CBH

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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