-
Plasmid#107156PurposeMBP-rPKL WT for production and purification of rat PKL WTDepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationPromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEE
Plasmid#159746PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 _34-78/myc-His
Plasmid#127496PurposeExpresses myc-tagged _34-78 NDUFS2 in mammalian cellsDepositorInsertNDUFS2 _34-78 (NDUFS2 Human)
UseTagsmyc-HisExpressionMammalianMutationNDUFS2 _34-78PromoterCMVAvailable sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-10-3xFLAG-LGP2-K651E
Plasmid#58685PurposeExpresses LGP2 with a mutation in the CTD (K651E) in mammalian cellsDepositorInsertLGP2-K651E (DHX58 Human)
UseTags3x FLAGExpressionMammalianMutationmutation to CTD, K651EPromoterCMVAvailable sinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lenti CGIP + M-AAT target
Plasmid#86008Purposelenti reporter plasmid with M-AAT-specific gRNA target-sequence, to be used with tGFP #26864DepositorInsertsCAG promoter
M-AAT target
UseLentiviralTagsExpressionMutationV30MPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging
Plasmid#217662Purposeexpresses Cas9-hGem and guideRNA for N terminal tagging of NIPBLDepositorInsertsgRNA3 for N terminal tagging of NIPBL (NIPBL Human)
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) T2C, C90A, T198C
Plasmid#220235Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationT2C, C90A, T198CPromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) C90A, T172C, T259C
Plasmid#220234Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationC90A, T172C, T259CPromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Creb5 shRNA
Plasmid#195020PurposeBovine Creb5 shRNA targeting the Creb5 3′UTRDepositorInsertCreb5 shRNA (CREB5 Bovine)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GAL4-Creb5 (1- 128) T59/T61 A
Plasmid#195032PurposeGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with T59/T61 A mutation)DepositorInsertGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with with T59/T61 A mutation) (CREB5 Bovine)
UseTagsExpressionMammalianMutationT59/T61 APromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GAL4-Creb5 (1- 128) C18/C23 S
Plasmid#195033PurposeGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation)DepositorInsertGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation) (CREB5 Bovine)
UseTagsExpressionMammalianMutationC18/C23 SPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-PARP1-del.p119K120S–eGFP
Plasmid#211554PurposeA piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette.DepositorInsertPARP1 (PARP1 Human)
UsePiggybacTagseGFPExpressionMammalianMutationdeletion of amino acids 119K 120SPromoterCMVAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-PARP1-delM43;F44I
Plasmid#211579PurposeCMV driven PARP1-delM43;F44I -eGFP expressing construct with IRES-Neomycin selectionDepositorInsertPARP1 (PARP1 Human)
UseTagseGFPExpressionMammalianMutationContains deletion of M43 and F44IPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFR*-CUbo-His6
Plasmid#212819PurposeConstitutive or doxycycline-inducible expression of EGFR*-CUbo-His6 in mammalian cellsDepositorInsertEGFR* (EGFR Budding Yeast, Human)
UseTagsCUbo-6xHisExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFR*-CUbo
Plasmid#212821PurposeConstitutive or doxycycline-inducible expression of EGFR*-CUbo in mammalian cellsDepositorInsertEGFR* (EGFR Budding Yeast, Human)
UseTagsCUboExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFR*-CUbo(K63R)
Plasmid#212822PurposeConstitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R) in mammalian cellsDepositorInsertEGFR* (EGFR Budding Yeast, Human)
UseTagsCUbo(K63R)ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+FLAG-KrasnatQ61R
Plasmid#206843PurposeTo express mouse Kras encoded by native mouse codons and with a Q61R mutation with an N-terminal FLAG tag.DepositorInsertKras (Kras Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Glutamine 61 to ArgininePromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G
Plasmid#204214PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (delDBD) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsExpressionMammalianMutationDeletion of DNA binding domain (delDBD)PromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G
Plasmid#204191PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (R162del) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsExpressionMammalianMutationDeletion of R162PromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G
Plasmid#204213PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsExpressionMammalianMutationPoint mutation on I207MPromoterTetONAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only