Addgene

We narrowed to 34,840 results for: lat

Showing: 9461 - 9480 of 34840 results

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pTK_Sox9_enh_349_Citrine

Plasmid

#130594

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox9_enh_349

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pQE60-EFTu-His

Plasmid

#124127

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Elongation factor Tu (tufB Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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3xERRE/ERE-luciferase

Plasmid

#37852

Depositor

Rebecca Riggins

Article

Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

Insert

3 copies of ERRE/ERE upstream of luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

SV40

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-RhoB

Plasmid

#23225

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

RhoB (Rhob Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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MET-HYGRO

Plasmid

#218113

Purpose

Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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LYS-Leu2

Plasmid

#218114

Purpose

Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pY3

Plasmid

#218116

Purpose

Activity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pY3-LYS-IV

Plasmid

#218119

Purpose

LYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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BFP-Rab5CA (Q79L)

Plasmid

#205705

Purpose

Mammalian expression of BFP-tagged mutant Rab5CA

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

RAB5A (RAB5A Human)

Use

Tags

Expression

Mammalian

Mutation

Q79L

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p23-DDX21 WT

Plasmid

#128803

Purpose

overexpression of DDX21 in human cells

Depositor

Ling-Ling Chen

Article

Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

Insert

DDX21 WT

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pgRNA-modified

Plasmid

#84477

Purpose

guide RNA expression lentiviral construct

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

guide RNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pJAT30

Plasmid

#204294

Purpose

For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-DsRed

Use

CRISPR

Tags

Expression

Mutation

Promoter

MHC

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-Sumo1

Plasmid

#21154

Purpose

Mammalian expression of human Sumo1 with HA tag

Depositor

Junying Yuan

Article

Terui et al J Biol Chem. 2004 Jul 2. 279(27):28257-6

Insert

pcDNA3-HA-Sumo1 (SUMO1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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pMPRAv3:minP-GFP

Plasmid

#109036

Purpose

MPRA v3 minimal promoter with GFP donor

Depositor

Ryan Tewhey, Pardis Sabeti

Article

Tewhey et al Cell. 2016 Jun 2;165(6):1519-29. doi: 10

Insert

emGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

minP

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Sp_PE

Plasmid

#212038

Purpose

Bacterial expression of the PE2 proteins for genome editing in E. coli

Depositor

Quanjiang Ji

Article

Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

Insert

PE2

Use

CRISPR

Tags

Expression

Mutation

Promoter

L-arabinose-inducible promoter PBAD

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_Keima

Plasmid

#127140

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3_Keima (RPS3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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V5-BS2-SENP2 (Nuclear Pore)

Plasmid

#220345

Purpose

Mammalian expression vector BS2 in nuclear pore

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Use

Tags

SENP2 (nuclear pore localized protein) and V5 tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMPRAv3:∆luc:∆xbaI

Plasmid

#109035

Purpose

MPRA v3 empty backbone

Depositor

Ryan Tewhey, Pardis Sabeti

Article

Tewhey et al Cell. 2016 Jun 2;165(6):1519-29. doi: 10

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

none

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLJM1 Flag Raptor

Plasmid

#26633

Depositor

David Sabatini

Article

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

raptor (RPTOR Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE25

Plasmid

#90367

Purpose

Notch - NIDC/CBF1 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Notch

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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