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Plasmid#72284PurposeExpress gene of interest under CMV promoter. WPRE has been removed to increase the cloning capacity.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 25, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits
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SV40-MCS-Z1
Plasmid#127639PurposeEmpty backbone plasmid with SV40 promoter and SV40 polyA with Zeocin/Bleocin bacterial resistanceDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterSV40 promoterAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJOE6087.3
Plasmid#135082PurposepBR322-based L-rhamnose-inducible vector.DepositorInsertP-rhaP-BAD-eGFP-His tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGLU_103
Plasmid#225785PurposeThis is the promoter region taken from upstream of Tn5 transposase in pBAM1. It can be considered the "default" promoter to use.DepositorInsertPtn5_[Tn5]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLU_85
Plasmid#225800PurposeThis is the promoter region taken from upstream of Himar transposase in pMarC9-Kan. It can be considered the "default" promoter to use.DepositorInsertPmarC9_[Himar]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBT1-proD-mCherry
Plasmid#65823PurposeConst. mCherry expression , proD promoter, E. coli ExpressionDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterproD E . coli Constitutive PromoterAvailable sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-OleD
Plasmid#59373PurposeInducible expression of OleD (DQ195536) under T7 promoterDepositorInsertOleD
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector
Plasmid#159281PurposeA multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)DepositorInsertAsCpf1-HA-2A-GFP
UseMulticistronic vector with both caggs promoter-dr…Tags3X HAExpressionMammalianMutationPromoterU6Available sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NurRE3
Plasmid#208253PurposepGL3 promoter luciferase reporter vector containing 3 copies of the NurRE POMC promoter DNA response element (5′-GATCGTGATATTTACCTCCAAATGCCA- 3′) used for luciferase reporter assays in mammalian cellsDepositorInsert3X Nur response element LUC
UseTagsNoExpressionMammalianMutationPromoterSV40 early promoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCK306
Plasmid#110544PurposerhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.DepositorInsertsPrhaBAD
yfp
rhaS
UseTagsExpressionBacterialMutationPromoterN/A, PrhaBAD, and kanR promoter (upstream of kanR…Available sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
PtPuc3_diaCas9_sgRNA
Plasmid#109219PurposeEpisome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutumDepositorInsertsLHCF2 promoter
diaCas9
LHCF1 terminator
U6 promoter
sgRNA
U6 3' region
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterLHCF1 terminator, LHCF2 promoter, U6 3' regi…Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
hGFAP-fLuc
Plasmid#40589DepositorInserthGFAP promoter (GFAP Human)
UseLuciferaseTagsFirefly luciferaseExpressionMutationContains human GFAP promoter fragment (-2163 to +…PromoterGFAP promoterAvailable sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
p2.1 (pAGL2A)
Plasmid#27563DepositorInsertENO1 promoter (ENO1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Pro E2
Plasmid#48749PurposeExpression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)DepositorInsertmPer2 promoter/enhancer region containing E-box2 (-112 to +98)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
scADGFP
Plasmid#114434PurposeEpilepsy promoter enhanced with Arc elements driving GFP with SV40E polyADepositorInsertGreen fluorescent protein
UseAAVTagsER export sequence FCYENEVExpressionMammalianMutationPromoterNovel synthetic activity-dependent promoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-MCK1.35lux
Plasmid#53368Purposeexpresses luciferase under 1.35 kb muscle creatine kinase promoter/enhancerDepositorInsertmuscle creatine kinase promoter/enhancer 1.35kb
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-24p3/LCN2-282kB mut-LUC
Plasmid#46867Purposemurine 24p3 minimal promoter with NF-kB mutation as a luciferase reporterDepositorInsert24p3/lipocalin 2 proximal promoter
UseLuciferaseTagsExpressionMutationNF- kB site is mutated (see comments)Promoter24p3 promoter (inactive)Available sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL3-24p3/LCN2-282C/EBP mut-LUC
Plasmid#46868Purposemurine 24p3 minimal promoter with C/EBP mutation k as a luciferase reporterDepositorInsert24p3/lipocalin 2 proximal promoter
UseLuciferaseTagsExpressionMutationCEBP site is mutated (see comments)Promoter24p3 promoter (inactive)Available sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
nes374tk/lacZ
Plasmid#47615Purposeused to create transgenic mice expressing LacZ reporter with Nestin enhancerDepositorInsertnestin 2nd intron fragment (NES Human)
UseEnhancer reporterTagsHSV tk promoter and lacZExpressionMutation374 bp from 2nd intron (comprising bp 1153 to 152…PromoterHSV tk promoterAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFIN-RK-GFP-mIRBP156-CHER-WPRE
Plasmid#44351DepositorInsertsRK-GFP
mIRBP156-CHER
UseLentiviralTagsExpressionMutationPromoterhuman rhodopsin kinase (pos 2554-2850) and mouse …Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only