Addgene

Showing: 921 - 940 of 2549 results

Results per page:

Sort results by:

pAAV.hSynapsin.TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR

Plasmid

#187101

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Pre-SPHERE-SF-iGluSnFR

Use

AAV

Tags

TagBFP-P2A

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCCL-PGK-SPdCas9-BFP-DNMT3B

Plasmid

#66820

Purpose

dCas9 fused to BFP and the human DNMT3B catalytic domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-DNMT3B, catalytic domain of DNMT3B

Use

Tags

dSpCas9-BFP-DNMT3B

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA27-pHR-MiniCMV-TriTag (mTagBFP)

Plasmid

#199458

Purpose

Expression of TriTag (mTagBFP) in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

TriTag

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

MiniCMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIP-SFFV-mtagBFP-2A RIGI K270A

Plasmid

#167290

Purpose

Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving catalytically dead (K270A) RLR sensor RIG-I

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

DDX58 (RIGI Human)

Use

Lentiviral

Tags

tagBFP

Expression

Mammalian

Mutation

K270A

Promoter

SFFV

Available since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MADR pDonor TagBFP2-V5-HRAS G12VWPRE

Plasmid

#129420

Purpose

MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion

Depositor

Joshua Breunig

Article

Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

Insert

TagBFP2-V5-HRAS G12V (HRAS Human, Entacmaea quadricolor (TagBFP))

Use

Mosaic analysis for dual recombinase-mediated cas…

Tags

TagBFP2-V5 tag

Expression

Mutation

G12V

Promoter

none (3x polyA to mitigate episomal expression)

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIP-SFFV-mtagBFP-2A STINGHAQ V155M

Plasmid

#102592

Purpose

Lentivector to express STING V155M HAQ and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

V155M R71H-G230A-R293Q

Promoter

SFFV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-FRT-EBFP-tTAn

Plasmid

#172127

Purpose

Expression of EBFP and tTA-InteinN in Flp recombinase positive cells

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

EBFP, tTAn-InteinN

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EK0396 BFPmut-stop-t70587 (pEAQ)

Plasmid

#191171

Purpose

EK0208 in plant-expressable format; Trigger 1 in 3' UTR of an inactive BFP

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

t70587 (plant)

Use

Tags

Expression

Plant

Mutation

WT

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS482-pCDest2-AcrIIC3Nme-mTagBFP2-IRES

Plasmid

#85750

Purpose

Expresses Type II-C anti-CRISPR protein, AcrIIC3Nme from Neisseria meningitidis, and mTagBFP2

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Type II-C anti-CRISPR AcrIIC3Nme
mTagBFP2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pNTKV- Esrrb-KI-3'UTR-2A-TagBFP-RBGpA

Plasmid

#128835

Purpose

Knock-in of TagBFP into mouse Esrrb locus

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

tagBFP into Esrrb locus (Esrrb Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2ABFP-W

Plasmid

#163178

Purpose

Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

YAP1 (YAP1 Human)

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCCL-PGK-SPdCas9-BFP-EGFP

Plasmid

#66821

Purpose

dCas9 fused to BFP and EGFP

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-EGFP

Use

Tags

dSpCas9-BFP-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS481-pCDest2-AcrE2-mTagBFP2-IRES

Plasmid

#85749

Purpose

Expresses anti-CRISPR protein from Type I-E and mTagBFP2

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Type I-E anti-CRISPR
mTagBFP2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6(BsmBI)-EF1a-BFP-Nkx2-5

Plasmid

#117135

Purpose

Expression vector for Nkx2-5 and gRNA

Depositor

Kosuke Yusa

Article

Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

Insert

Nkx2-5 CDS (Nkx2-5 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

modified BFP

Promoter

human U6 promoter, human EF1a promoter

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-CAG-F14F15S-BFP2-(mCherry)'

Plasmid

#115235

Purpose

Lentiviral transfer plasmid for expression of mTagBFP2 before Flp recombination and mCherry after it

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

mTagBFP2 and mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUFlip-floxed2A-KalTA4-; gcry1:BFP -0

Plasmid

#173889

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; gcry1: BFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAT15416-BEAR-mScarlet-target-BFP

Plasmid

#162996

Purpose

plasmid expressing an sgRNA targeting the BEAR-mScarlet plasmid along with a TagBFP marker

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

sgRNA targeting the BEAR-mScarlet plasmid

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS507-pCDest2-noAcr-mTagBFP2-IRES

Plasmid

#85748

Purpose

Expresses mTagBFP2 only; used as a control

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

mTagBFP2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUFlip-floxed2A-KalTA4-; gcry1:BFP -1

Plasmid

#173890

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; gcry1: BFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-BFP-pA_U6-BbsI (pE058)

Plasmid

#149352

Purpose

Mammalian expression vector for BFP

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge